| Beclin 1在乳腺浸润性导管癌中的表达及其与Ki67的关系 |
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| 引用本文： | 宦宇,蔡徐山,焦家军,吴军录,权文强,张春利,席芳. Beclin 1在乳腺浸润性导管癌中的表达及其与Ki67的关系[J]. 检验医学, 2019, 0(7): 595-599 |
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| 作者姓名： | 宦宇 蔡徐山 焦家军 吴军录 权文强 张春利 席芳 |
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| 作者单位： | 上海市嘉定区妇幼保健院检验科;上海市嘉定区妇幼保健院乳腺科;同济大学附属同济医院检验科;上海市嘉定区妇幼保健院病理科 |
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| 基金项目： | 上海市嘉定区卫生和计划生育委员会资助项目(QNKYJJ 2015-QN-04) |
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| 摘 要： | 目的探讨自噬相关基因Beclin 1和增殖相关基因Ki67编码的蛋白在乳腺浸润性导管癌中的表达情况。方法选取乳腺浸润性导管癌患者64例。采用免疫组化法检测癌组织及对应的癌旁组织中Beclin 1和Ki67蛋白的表达,同时采用免疫荧光法和免疫印迹法检测癌组织及对应癌旁组织中Beclin 1蛋白的表达。结果癌组织中Beclin 1的阳性表达率(43.8%)明显低于癌旁组织(98.5%)(P<0.05),而Ki67的阳性表达率(76.6%)则高于癌旁组织(0.00%)(P<0.05)。癌组织中Beclin 1蛋白表达与Ki67蛋白表达呈负相关(r=-0.256,P<0.05)。Beclin 1蛋白表达与乳腺浸润性导管癌病理分级、有无淋巴结转移有关(P<0.05),与肿瘤直径、年龄、雌激素受体(ER)、孕激素受体(PR)、人类表皮生长因子受体2(HER-2)均无关(P>0.05)。Ki67蛋白表达与乳腺浸润性导管癌病理分级、肿瘤直径有关(P<0.05),与有无淋巴结转移、年龄、ER、PR、HER-2均无关(P>0.05)。免疫荧光法检测结果显示癌组织中Beclin 1蛋白的阳性表达率(30.0%)明显低于癌旁组织(100.0%)(P<0.05)。免疫印迹法检测结果显示癌旁组织中Beclin l蛋白的相对表达量为0.43±0.12,明显高于癌组织(0.20±0.18)(P<0.05)。结论乳腺浸润性导管癌组织中Beclin 1蛋白表达明显降低,Ki67蛋白表达明显升高。
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| 关 键 词： | BECLIN 1基因 KI67基因 细胞自噬 细胞增殖 乳腺浸润性导管癌 |
Expression of Beclin 1 in breast invasive ductal carcinoma and its relationship with Ki67 |
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| HUAN Yu,CAI Xushan,JIAO Jiajun,WU Junlu,QUAN Wenqiang,ZHANG Chunli,XI Fang. Expression of Beclin 1 in breast invasive ductal carcinoma and its relationship with Ki67[J]. Laboratory Medicine, 2019, 0(7): 595-599 |
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| Authors: | HUAN Yu CAI Xushan JIAO Jiajun WU Junlu QUAN Wenqiang ZHANG Chunli XI Fang |
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| Affiliation: | (Department of Clinical Laboratory,Maternal and Child Health Hospital of Jiading District,Shanghai 201821,China;Galactophore Department,Maternal and Child Health Hospital of Jiading District,Shanghai 201821,China;Department of Clinical Laboratory,Tongji Hospital,Tongji University,Shanghai 200065,China;Pathology Department, Maternal and Child Health Hospital of Jiading District,Shanghai 201821,China) |
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| Abstract: | HUAN Yu;CAI Xushan;JIAO Jiajun;WU Junlu;QUAN Wenqiang;ZHANG Chunli;XI Fang(Department of Clinical Laboratory,Maternal and Child Health Hospital of Jiading District,Shanghai 201821,China;Galactophore Department,Maternal and Child Health Hospital of Jiading District,Shanghai 201821,China;Department of Clinical Laboratory,Tongji Hospital,Tongji University,Shanghai 200065,China;Pathology Department, Maternal and Child Health Hospital of Jiading District,Shanghai 201821,China) |
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| Keywords: | Beclin 1 Ki67 Cell autophagy Cell proliferation Breast invasive ductal carcinoma |
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