NMDA-induced increase in [Ca]i andCa uptake in acutely dissociated brain cells derived from adult rats |
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Authors: | Martin Villalba Alberto Martinez-Serrano Cristina Bo
rner Pablo Blanco Jorgina Satrustegui |
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Institution: | Marti´n Villalba, Alberto Marti´nez-Serrano, Cristina Bo¨rner, Pablo Blanco,Jorgina Satru´stegui, |
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Abstract: | A preparation of acutely dissociated brain cells derived from adult (3-month-old) rat has been developed under conditions preserving the metabolic integrity of the cells and the function of N-methyl-d-aspartate (NMDA) receptors. The effects of glutamate and NMDA on Ca2+]i measured with fluo3 and45Ca2+ uptake have been studied on preparations derived from hippocampus and cerebral cortex. Glutamate (100 μM) and N-methyl-dl-aspartate (200 μM) increased Ca2+]i by 26-12 nM and 23-9 nM after 90 s in cerebral cortex and hippocampus, and stimulated45Ca2+ uptake about 16–10% in the same regions. The increases in Ca2+]i and45Ca2+ uptake were inhibited by 40% in the presence of 1 mM MgCl2 and by 90–50% in the presence of MK-801. The results indicate (a) that a large fraction of the Ca2+]i response to glutamate in freshly dissociated brain cells from the adult rat involves NMDA receptors, (b) when compared with results in newborn rats, there is a substantial blunting of the Ca2+]i increase in adult age. |
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Keywords: | Dissociated brain cell Intracellular free calcium Fluo3 45Ca2+ uptake N-Methyl-d-aspartate |
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