PAG内微量注射OFQ并同时电针对脊髓背角单胺类和氨基酸类递质释放的影响

我们以往的工作表明 ,大鼠中脑导水管周围灰质 (ＰＡＧ)内微量注射孤啡肽 (ＯＦＱ)对抗针刺镇痛 ;并使脊髓背角谷氨酸 (Ｇｌｕ)释放增加 ,ν 氨基丁酸 (ＧＡＢＡ)、5羟色胺 (5 ＨＴ)、去甲肾上腺素(ＮＥ)释放减少 ;脊髓背角广动力范围 (ＷＤＲ)神经元的Ｃ 纤维反应和后放电增多。本研究的目的是采用核团内注射、微透析加高效液相电化学检测的方法 ,观察ＰＡＧ内微量注射ＯＦＱ并同时电针 ,对大鼠脊髓背角单胺类和氨基酸类递质释放的影响。结果显示 ,ＰＡＧ内微量注射ＯＦＱ并同时电针后 ,脊髓背角Ｇｌｕ含量与相应时间点的ＯＦＱ组相比 ,在 …

Effect of Microinjection of OFQ into PAG and Simultaneous Electroacupuncture on Release of Monoamines and Amino Acids in Spinal Dorsal Horn

Our previous work demonstrated that microinjection of OFQ into PAG antagonized e lectroacupuncture (EA) analgesia; increased the release of Glu in spinal dorsal horn and decreased the release of GABA, 5 HT and NE; and significantly facilita t ed c fibre response and post discharge of WDR neurons of spinal dorsal horn in t he rats. The aim of current study is to observe the effect of microinjection of OFQ into PAG and simultaneous EA on the release of monoamines and Amino acids in spinal dorsal horn in the rats, by the methods of nucleus injection, micro dia lysis and HPLC EC. The results were as follows: After microinjection of OFQ into PAG and simultaneous EA, extracellular concentr ation of Glu was significantly decreased, whereas extracellular concentratio ns of GABA, 5 HT and 5 HIAA, NE and MHPG were significantly increas ed (P<0.05～0.01), indicating significant differences in comparison wi t h OFQ control group (microinjection of OFQ into PAG alone) in the corresponding phases (P<0.5～0.001). The results suggest there are opposite effects on the descending pain modulating system between OFQ in the brain and EA, so this may be one of the mechanisms of OFQ in the brain antagonizing EA analgesia.