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ATR-FTIR analysis of protein adsorption on polymeric surfaces
Abstract:ATR-FTIR spectroscopy was used to study the adsorption of bovine serum albumin on poly(vinyl chloride), poly(vinylidene fluoride), poly(methyl methacrylate), and poly(ethylene glycol)-grafted poly(methyl methacrylate) surfaces. The results were analyzed in terms of the amount of albumin adsorbed on the various polymeric surfaces when immersed in a 1 g/l protein solution. Experimental results were compared with the Van Straaten and Peppas model (1990) and satisfactory agreement was obtained. It was confirmed that poly(ethylene glycol) surfaces exhibited the lowest albumin adsorption patterns.
Keywords:ATR-FTIR spectroscopy  adsorption kinetics  bovine serum albumin  poly(vinyl chloride)  poly(vinylidene fluoride)  poly(methyl methacrylate)  poly(ethylene glycol)-grafted poly(methyl methacrylate) surfaces
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