心肌细胞蛋白质合成与活力的关系

目的 观察不同药物干预下,新生大鼠心肌细胞蛋白含量与活力的关系.方法 采用考马斯亮蓝法检测蛋白含量,MTT比色法测量心肌细胞的A490值,以A490/总细胞数之比作为衡量心肌细胞活力的指标.结果 心肌细胞蛋白含量随着ET-1的浓度增大而增加,ET-1引起蛋白含量的增加可被拉西地平和川芎嗪减弱,心肌细胞的A490/总细胞数之比随着ET-1的浓度增加而降低,拉西地平和川芎嗪也可进一步降低心肌细胞的A490/总细胞数之比.结论 心肌细胞蛋白质含量的增加并不引起细胞活力的增强,表明心肌细胞蛋白含量与活力之间可能缺乏相关关系.

Relationship between cardiomyocyte protein synthesis and cell viability

Objective To observe the relationship between protein sythesis and cardiomyocyte viability in neonatal rats. Methods The protein sythesis in neonatal rat cardiomyocytes was measured according to Brandford's method, the absorbance at 490 nm (A490 nm) of the cells was measured with MTT assay and the cell viability evaluated by the ratio of A490 nm to the total cell number. Results ET-1 increased cardiomyocyte protein synthesis dose-dependently, and this effect was attenuated by the application of larcidipine and tetramethylpyrazines Higher doses of ET-1 resulted in lower A490 nmtotal cell number ratio, which was further lowered by larcidipine and tetramethylpyrazine. Conclusion The status of protein synthesis is not associated with the viability of neonatal rat cardiomyocytes.