| 利用RAPD标记分析长白山越桔的遗传多样性及亲缘关系 |
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| 引用本文： | 王程,罗军,田志杰,姜艳霞,李妍,许娜,芦晓晶,吕士杰.利用RAPD标记分析长白山越桔的遗传多样性及亲缘关系[J].吉林医药学院学报,2009,30(2). |
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| 作者姓名： | 王程 罗军 田志杰 姜艳霞 李妍 许娜 芦晓晶 吕士杰 |
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| 作者单位： | 吉林医药学院生物化学教研室,吉林,吉林132013 |
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| 基金项目： | 吉林省教育厅科学技术研究基金 |
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| 摘 要： | 目的分析长白山越桔遗传多样性及其亲缘关系。方法用随机扩增多态DNA(RAPD)标记方法对1种野生越桔和2种不同的栽培越桔进行遗传多样性检测和遗传分析。结果采用40个随机引物共检测72个位点,其中多态位点27个,占37.5%;相似系数矩阵表明野生越桔和栽培越桔Ⅰ、栽培越桔Ⅱ的遗传距离分别为0.764和0.809;聚类图表明野生越桔和栽培越桔Ⅱ亲缘关系较近。结论长白山越桔的遗传多样性水平较低,野生越桔和栽培越桔之间的遗传变异不大,遗传因素在越桔形态变异上的作用小于环境因素。
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| 关 键 词： | 越桔 随机扩增多态DNA 遗传多样性 |
Genetic diversity analysis of blueberry from the Changbai Mountain by RAPD marker |
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| WANG Cheng,LUO Jun,TIAN Zhi-jie,JIANG Yan-xia,LI Yan,XU Na,LU Xiao-jing,LV Shi-jie.Genetic diversity analysis of blueberry from the Changbai Mountain by RAPD marker[J].Journal of Jilin Medical College,2009,30(2). |
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| Authors: | WANG Cheng LUO Jun TIAN Zhi-jie JIANG Yan-xia LI Yan XU Na LU Xiao-jing LV Shi-jie |
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| Institution: | Department of Biochemistry;Jilin Medical College;Jilin City;Jilin Province;132013;China |
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| Abstract: | Objective Analyzes genetic diversity and sibship of blueberry from the Changbai Mountain.Methods Genetic diversity examination and the genetic analysis to one kind of wild blueberry and two kind of different garden blueberry by random amplified polymorphic DNA(RAPD)marker.Results From the forty random primers 72 sites were detected,of which 27(37.5%)were polymorphism.The Proximity Matrix indicates Genetic Proximity Values between the wild blueberry and the garden blueberryⅠ,the garden blueberryⅡrespectively... |
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| Keywords: | blueberry RAPD genetic diversity |
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