针刺对应激状态下小鼠细胞免疫功能影响的实验研究

目的 :揭示任脉六穴与胸腺的内在联系 ,探讨针灸对应激状态下小鼠细胞免疫功能的调节作用及机理。方法 :通过长期的捆绑刺激使小鼠处于应激状态 ,分别采用手针和电针刺激小鼠的任脉六穴 ,观察针刺前后NK细胞活性、白细胞介素 2活性的变化。结果 :应激组中NK细胞活性和白细胞介素 2活性均低于对照组 (P <0 .0 5,P <0 .0 1 ) ,而手针组、电针组与应激组相比NK细胞的活性和白细胞介素 2活性均得到提高 (P <0 .0 1 ,P <0 .0 5)。结论 :针刺任脉六穴可以提高应激状态下小鼠的细胞免疫功能 ,其作用机制主要是通过对胸腺的影响 ,进而提高小鼠的NK细胞活性和白细胞介素 2的活性 ,从而显现出针刺的促防卫免疫效应

Effect of Acupuncture on the Cellular Immune Function of the Rat under Stress State

Objective: To observe the effect of acupuncture of 6 acupoints of the Conception Vessel (CV) on the activities of NK cells and interleukin(IL)-2. Methods: NIH mice were randomized into control group (n=15), stress group (n=15), handle-needling group (n=15) and electroacupuncture (EA) group (n=15). "Danzhong"(CV 17), "Yutang"(CV 18), "Zigong"(CV 19), "Huagai"(CV 20), "Xuanji"(CV 21) and "Tiantu"(CV 22) were punctured with acupoint to acupoint penetration method and stimulated for 20 min. The treatment was conducted once daily, with 10 days being a therapeutic course and with the interval between two courses being one week. Following 3 courses of treatment, the mouse was killed for sampling spleen and thymus tissues. NK cell and IL-2 activities in cell suspension of the spleen and thymus were detected. Results: In stress group, both NK cell and IL-2 activities were significantly lower than those of control group, while those of handle-needling and EA groups were apparently higher than those of stress group (P<0.05～ 0.01), suggesting that acupuncture can raise NK cell and IL-2 activities in stress mice. Conclusion: Both handle-needling and electroacupuncture can enhance activities of NK cells and IL-2 in stress mice.