| 自然诱变品种红金银花中木犀草苷含量初探 |
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| 引用本文： | 靳维荣,李广华,孙强,单成钢,王志芬.自然诱变品种红金银花中木犀草苷含量初探[J].现代中药研究与实践,2011(5):22-24. |
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| 作者姓名： | 靳维荣 李广华 孙强 单成钢 王志芬 |
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| 作者单位： | 山东省农业科学院农产品研究所/药用植物研究中心;山东省聊城市药品检验所;山东省泰安市药品检验所; |
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| 基金项目： | 农业部公益性行业科研项目“核技术农业应用”(200803034) |
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| 摘 要： | 目的 应用HPLC对自然突变品种红金银花中化学成分进行初步分析,建立了其中木犀草苷的分析方法并测定其含量.方法 选用Venusil XBP-C18柱,以乙腈-磷酸-水为流动相进行梯度洗脱,采用Agilent DAD检测器(238 nm)对自然诱变品种红金银花中木犀草苷进行了初步分析测定.结果 木犀草苷的保留时间为39....
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| 关 键 词： | HPLC 红金银花 木犀草苷 |
The Analysis of Luteolin Content in Variety of Natural Mutation-Lonicera japonica var.chinensis |
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| JIN Wei-rong,Li Guang-hua ,SUN Qiang ,SHAN Cheng-gang,WANG Zhi-fen.The Analysis of Luteolin Content in Variety of Natural Mutation-Lonicera japonica var.chinensis[J].Research and Practice on Chinese Medicines,2011(5):22-24. |
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| Authors: | JIN Wei-rong Li Guang-hua SUN Qiang SHAN Cheng-gang WANG Zhi-fen |
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| Institution: | JIN Wei-rong1,Li Guang-hua 2,SUN Qiang 3,SHAN Cheng-gang1,WANG Zhi-fen1(1.Institute of Agro-Food Science &Technology,Shandong Academy of Agricultural Sciences/Herbal medicine research center,Jinan 250100,China,2.Liaocheng Institute for Drug Control,Liaocheng 252000,3.Taian Institute for Drug Control,Taian 271000,China) |
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| Abstract: | Objective To establish an HPLC method for the analysis of chemical constituent and determined the content of luteolin in variety of natural mutation-Lonicerajaponica var chinensis. Methods HPLC analysis was performed on Venusil XBP - C18 （ 250 mm×4.6 mm, 5 μm ） column with mobile phase of acetonitrilephosphoric acid-water and the detection wavelength was 238 nm. Results The retention time of luteolin was 39.50 min, the linear range was 0.035 5 - 0.284 0 μg （ r=0.999 9 ）.The RSD of reproducibility and stability experiments was lower than 3% and the average recovery rate of luteolin is 98.85%.Conclusion This method was simple and fast with more accuracy and reproduciblity to detect luteolin in Lonicerajaponica var. chinensis. The average content of luteolin in Lonicerajaponica var. chinensis was 0.54mg/g. |
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| Keywords: | HPLC Lonicera japonica var chinensis luteolin |
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