| HPLC梯度洗脱法测定山栀苷甲酯、8-0-乙酰山栀苷甲 酯在独一味中含量 |
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| 引用本文： | 廖立东,唐灿,卢胜明,张康宁,邓艳,屠鹏飞.HPLC梯度洗脱法测定山栀苷甲酯、8-0-乙酰山栀苷甲 酯在独一味中含量[J].现代中药研究与实践,2011(5):70-72. |
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| 作者姓名： | 廖立东 唐灿 卢胜明 张康宁 邓艳 屠鹏飞 |
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| 作者单位： | 成都中医药大学;甘肃独一味生物制药股份有限公司;西华大学;北京大学药学院; |
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| 摘 要： | 目的 建立独一味中山栀苷甲酯、8-O-乙酰山栀苷甲酯的HPLC检测方法.方法 色谱柱Dikma platisil C18( 250 mm×4.6 mm,5 μm),柱温35℃,以乙腈-水进行梯度洗脱,流速1.0 mL/min,检测波长235 nm.结果 山栀苷甲酯进样量在0.023 0～3.450 0 μg范围内呈良好...
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| 关 键 词： | 独一味 山栀苷甲酯 8-O-乙酰山栀苷甲酯 高效液相色谱法 含量测定 |
Determination of Shanzhiside Methyl Ester and 8-O-acetylshanzhiside Methyl Ester in Lamiophlomis rotate(Benth.) Kudo by HPLC |
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| LIAO Li-dong,TANG Can,LU Sheng-ming,ZHANG Kang-ning,DENG Yan,TU Peng-fei.Determination of Shanzhiside Methyl Ester and 8-O-acetylshanzhiside Methyl Ester in Lamiophlomis rotate(Benth.) Kudo by HPLC[J].Research and Practice on Chinese Medicines,2011(5):70-72. |
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| Authors: | LIAO Li-dong TANG Can LU Sheng-ming ZHANG Kang-ning DENG Yan TU Peng-fei |
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| Institution: | LIAO Li-dong1,2,TANG Can3,LU Sheng-ming2,ZHANG Kang-ning2,DENG Yan2,TU Peng-fei4 (1.Chengdu University of TCM,Chengdu 610075,China,2.Gansu Duyiwei Bio-Phanmaceutical Inc.,Chengdu 610063,3.Xihua University,Chengdu 610039,4.Peking University,School Pharmaceutical Sciences,Beijing 100191,China) |
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| Abstract: | Objective To establish a method to determine Shanzhiside methyl ester and 8-O-acetylshanzhiside methyl ester in LamiophIomis rotate （Benth.） Kudo by HPLC. Methods HPLC analysis was performed on Dikma platisil C18 （ 250 mm×4.6 mm, 5 μm） column with gradient elution of acetonitrile-water at a flow rate of 1.0mL/min. The column temperature was 30℃and the detection wavelength was 235nm. Results The linear range of Shanzhiside methyl ester was 0.023 0-3.450 0 μg, r-=0.999 3. The linear range of 8-O-acetylshanzhiside methyl ester was 0.041 82-6.273 μg, r=0.999 6.The average recovery of Shanzhiside methyl ester was 99.79% with RSD 1.63%, the average recovery of 8-O-acetylshanzhiside methyl ester was 99.62% with RSD 1.79%. Conclusion The method is sentitive, rapid and reproducible. |
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| Keywords: | Lamiophlomis rotate(Benth ) Kudo Shanzhiside methyl ester 8-O-acetylshanzhiside methyl ester HPLC Determination |
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