伊贝沙坦对兔心肌缺血/再灌注P-选择素表达的影响

目的探讨伊贝沙坦对兔心肌急性缺血、再灌注损伤和缺血、再灌注区P－选择素表达的影响。方法假手术组左冠状动脉前降支近端穿线但不结扎，另两组则结扎60min后，松开结扎线，予以360min再灌注。处死动物后，从再灌注区取下组织作标本，予以常规组织学处理。组织HE染色后，光镜下检查，用免疫组化SP方法检测P－选择素在标本组织中的表达情况。结果与对照组相比，治疗组的组织损伤和中性粒细胞渗出程度明显减少（P〈0．01、P〈0．01）；假手术组更低。对照组和治疗组再灌注区P－选择素表达明显上调（P〈0．01，vs假手术组）；但两组上述指标之间有显著差异，前者P－选择素表达更强（P〈0．01）。结论心肌急性缺血、再灌注诱导再灌注区P－选择素表达上调；伊贝沙坦能明显抑制P－选择素表达上调；对兔心肌急性缺血、再灌注损伤有重要的保护作用。

Effect of irbesartan on expression of P-selection in the myocardial ischemia-reperfusion areas of rabbits

Objective The aim was to explore the effects provided by irbesartan on acute myocardia ischemial-reperfusion injury and on the expression of P-selection in the myocardial ischemia-reperfusion areas.Methods In the sham group,left coronary artery was not ligated.In the other two groups,the rabbits were subjected to 60 min of left coronary artery occlusion,followed by 360 min of reperfusion.The rabbit was killed.The tissue samples removed from I/R areas.The samples were disposed with routine histological methods.They were examined by light microscopy after staining with hematoxylin and eosin(HE).Also,the expressions of P-selection were investigated by employing immunohistochemical SP method in the tissue samples.Results As compared with the control group,the damage degree of myocytes and neutrophil infiltration were greatly reduced in the treated group (P<0.01;P<0.01),and got more lower in the sham group.Expression of P-selection in the I/R areas both in the control group and in the treated group were greatly regulated (P<0.01,vs sham group).And the expressions of above in vestigated index were significantly different between the control group and the treated group,in the former,the expression of P-selection tended to be more strong (P<0.01).Conclusion These data suggest that (1) I/R markely induced expression of P-selection in the I/R areas.(2)irbesartan can greatly depressed expression of P-selection in the I/R areas.(3)irbesartan exerts an important protective effect against myocardial I/R injury of rabbits.