| 一种新的蝮蛇蛇毒磷脂酶A2同源物的分离纯化及其氨基酸序列分析 |
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| 引用本文： | 马安德,吴少瑜,张嘉杰,李志琴,徐伟,余乐,文晓芸,吴曙光. 一种新的蝮蛇蛇毒磷脂酶A2同源物的分离纯化及其氨基酸序列分析[J]. 中药材, 2006, 29(2): 132-134 |
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| 作者姓名： | 马安德 吴少瑜 张嘉杰 李志琴 徐伟 余乐 文晓芸 吴曙光 |
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| 作者单位： | 1. 南方医科大学分析测试中心,广东,广州,510515
2. 南方医科大学药学院,广东,广州,510515 |
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| 摘 要： | 采用C18反相高效液相色谱法从蛇毒粗毒中提取出一种新的PLA2同源物,其分子量为13900Da,N末端氨基酸序列分析结果为:HLLQFRKM IKKMTKK。通过GenBank的prote in-prote in BLAST软件进行同源性分析,结果表明,N端的15个氨基酸与已知其他蛇毒PLA2具有较高的同源性。
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| 关 键 词： | 蝮蛇 蛇毒 PLA2同源物 氨基酸序列 |
| 文章编号： | 1001-4454(2006)02-0132-03 |
| 收稿时间： | 2005-07-18 |
| 修稿时间： | 2005-07-18 |
Purification of A New Phospholipase A2 Homologue from Agkistrodon blomhoffiiSiniticus and Its Effects on Gene Expression of Hep3B Cells |
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| MA An-de,WU Shao-yu,ZHANG Jia-jie,LI Zhi-qin,XU Wei,YU Le,WEN Xiao-yun,WU Shu-guang. Purification of A New Phospholipase A2 Homologue from Agkistrodon blomhoffiiSiniticus and Its Effects on Gene Expression of Hep3B Cells[J]. Journal of Chinese medicinal materials, 2006, 29(2): 132-134 |
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| Authors: | MA An-de WU Shao-yu ZHANG Jia-jie LI Zhi-qin XU Wei YU Le WEN Xiao-yun WU Shu-guang |
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| Affiliation: | 1. lnstnlmental Analysis and Re, search Center Sot, them Medical University, Guangzhou 510515,China; 2. College of Pharmacological Sciences Southern Medical University, Guangzhou 510515,China |
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| Abstract: | A new PLA2 homologue was purified from Agkistrodon blomhoffii Siniticus by applying reverse phase(HPLC) C_(18) column.The molecular weight of PLA2 homologue is 13900Da and its purity is 97.2%.The results of N-terminal sequence analysis were HLLQFRKMIKKMTKK.All the fragments were determined with the protein-protein BLAST software of GenBank.BLAST analysis showed that the N-terminal sequences of 15 amino acids were highly homologous with the sequences of other PLA2. |
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| Keywords: | Agkistrodon blomhoffii Siniticus Snack venom PLA2 homologue Amino acid sequence |
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