吡喹酮抗日本血吸虫雄虫过程中免疫血清的作用

体内或体外培养的血吸虫雄虫经一定浓度的吡喹酮作用后,移置含免疫血清的培养液中培养时,受损害的虫体体表有大量絮粒状物沉积,形成膜状包被,加重皮层的损害;在继续培养3天时,大部分或全部虫未见有明显恢复。若将虫移置含正常血清的培养液中培养时,则无此种现象,且大部虫的体表损害有不同程度的恢复。上述结果亦为扫描电镜的观察所证实。

THE EFFECT OF IMMUNE SERUM ON MALE SCHISTOSOMA JAPONICUM SUBJECTED TO PYQUITON

When male worms of Schistosoma japonicum were maintained in culture medium containing immune rabbit serum (IRS) and exposed to pyquiton, numerous granular flocculous materials deposited on the drug-damaged surface of the worms. The flocculous materials aggregated together to form a membrane-like sheath around the worm accentuating the tegument injury. If IRS in the medium was replaced by normal rabbit serum (NRS), no such phenomenon was observed. When male schistosomes were exposed to pyquiton at a concentration of 30 μg/ml for 1 or 4 h, then transferred to the medium containing NRS and incubated for another 3 days, the damaged tegument and worm activity recovered apparently in about one half of the worms tested. However, if the male schistosomes were transferred to the medium containing IRS, most or all of the worms showed no significant recovery. In another experiment, male schistosomes, perfused out from infected mice 4～8 h after oral administration of pyquiton at a single dosage of 400 mg/kg, were transferred to the medium containing IRS and incubated for another 3 days, no apparent recovery of tegument damage nor worm activity was detected in most or all of the worms tested, while transferred to the medium containing NRS exhibited recovery in different degree. The results were also confirmed by scanning electron microscopic observation.