硫磺熏蒸前后党参红外光谱特征变化规律

目的:研究硫磺熏蒸前后党参红外光谱的特征变化规律,为党参药材的质量控制提供鉴别依据。方法:对党参药材以不同比例的硫磺进行熏蒸,采用傅里叶变换红外光谱法对熏蒸前后的党参药材、党参醇提物和党参水提物进行红外图谱及二阶导数图谱分析。结果:对于党参药材、党参醇提物和党参水提物3种分析对象来说,其各自的未熏党参与硫磺熏蒸党参在红外光谱上的差别并不明显,而通过具有高分辨力的二阶导数光谱分析后,可得到明显的差别变化。二阶导数光谱显示,对于党参药材,硫磺熏蒸前后的吸收峰在1 500~1 600 cm-1峰强变化比较明显,而对于党参醇提物和水提物来说,在1 500~1 600,1 715 cm-1处峰强变化明显,且随熏蒸党参硫磺用量的不同峰强有各自的变化规律。结论:利用硫磺熏蒸前后党参红外光谱的变化规律,可快速、高效地鉴别党参是否经过硫磺熏蒸处理,为党参药材的质量控制提供鉴别依据。

Change Regulation on Infrared Fingerprint Spectra of Codonopsis Radix Before and After Sulfur-fumigated Process

Objective: Study the change regulation on FTIR spectra of Codonopsis Radix before and after sulfur-fumigated process, and provide a basis for quality control of Codonopsis Radix. Method: Different quantities of sulfur were used to fumigate Codonopsis Radix. The powder, alcoholic and aqueous extracts of Codonopsis Radix before and after sulfur-fumigated processes were analyzed by FTIR, the further analysis was used by second derivative infrared spectroscopy. Result: Before and after sulfur-fumigated process, the difference of FTIR spectra was not obvious, while through high resolution of second derivative infrared spectroscopy the distinction was showed. The messages of infrared spectra are as follows:for powder of Codonopsis Radix, the height of absorption peaks in the range of 1 500-1 600 had obvious difference. And for alcoholic extract and aqueous extract, the same feature had appearance in the range of 1 500-1 600 cm^-1, 1 715 cm^-1. The height of absorption peaks of powder of Codonopsis Radix had a regular change along with the quantity of sulfur. Conclusion: The change regulation on FTIR spectra before and after sulfur-fumigated process could be utilized to distinguish the Codonopsis Radix whether or not to be sulfur-fumigated, and provide a basis for quality control.