补心软脉颗粒对麻醉犬急性心肌缺血的保护作用

目的:研究补心软脉颗粒对实验性犬急性心肌缺血的保护作用。方法:将36只实验犬随机分成补心软脉颗粒低、中、高剂量(7.33,14.67,29.33 g·kg-1)组、心元胶囊组、假手术组和模型组,除假手术组外各组均通过手术结扎犬冠状动脉左前降支(LAD)造成急性心肌梗死模型,各组动物于手术结扎后经十二指肠给药。观察各组犬心电图、心脏冠脉流量、心膜电位和血清酶的变化;硝基蓝四唑(NBT)组织化学染色观察心肌梗死面积;血气分析仪测量动静脉含氧量。结果:模型组心肌耗氧量、心肌梗死面积、肌酸激酶(CK)和乳酸脱氢酶(LDH)的活性均高于假手术组(P0.05,P0.01);补心软脉颗粒中、高剂量组成剂量依赖性可显著地降低心肌缺血犬的心肌耗氧量,缩小心肌梗死范围及梗死程度,降低血清CK和LDH的活性,增加冠脉流量(P0.05,P0.01),且补心软脉颗粒高剂量组上述指标的变化较其他各组更为明显(P0.01)。结论:补心软脉颗粒通过减少心肌耗氧量,降低梗死面积,对缺血心肌具有保护作用。

Protective Effects of Buxin Ruanmai Granules on Acute Myocardial Ischemia in Anaesthetized Dogs

Objective: To observe the protective effects of Buxin Ruanmai granules on acute myocardial infarction in anaesthetized dogs. Method: A total of 36 dogs were divided into the Buxin Ruanmai granules high dose, middle dose,low dose groups,Xinyuan capsule group,model group and sham group.The hemodynamics and myocardial oxygen metabolism, the myocardial infarct size,and the changes of the enzymes in serum were determined in the acute myocardial infarction model induced by ligation of left anterior descending (LAD) in the anaesthetized dogs;nitro blue tetrazolium (NBT) staining was used to observe infarct size;blood gas analyzer was used to measure oxygen content. Result: Except for increasing coronary blood flow,the cardium oxygen consumption, myocardial infarct size, the activity of serum creatine kinase (CK) and lactate dehydrogenase (LDH) were decreased in acute myocardial infarction dogs treated with the Buxin Ruanmai granules(P<0.05, P<0.01). Buxin Ruanmai high dose had significant difference(P<0.01). Conclusion: The Buxin Ruanmai granules have protective effects on myocardial ischemia in anaesthetized dogs.