白术多糖对自发性2型糖尿病小鼠血糖及相关指标的影响

目的:观察白术多糖对自发性2型糖尿病db/db小鼠的降血糖作用。方法:以db/db 2型糖尿病小鼠为研究对象,分为模型组、阳性药组(盐酸二甲双胍250 mg·kg-1)、白术多糖3个剂量组(300,90,30 mg·kg-1),连续ig给予相应药物4周。给药期间观察小鼠的一般体征及体重,于给药第2,4周检测空腹血糖(FBG);给药4周后,检测小鼠空腹血浆胰岛素(Ins)以及口服糖耐量(OGTT)的变化,并根据血糖计算胰岛素敏感性指数(ISI)。结果:与模型组相比,白术多糖(300,90 mg·kg-1)给药期间db/db小鼠整体状态较好,毛色较光滑,饮食饮水量降低,明显降低db/db小鼠空腹血糖、血浆胰岛素,升高胰岛素敏感性指数,降低小鼠在给予葡萄糖后各个时间点(0.5,2 h)血糖值及血糖曲线下面积(AUC),(P0.05,P0.01);但对体重无明显影响。结论:白术多糖能够有效降低db/db 2型糖尿病小鼠的空腹血糖,降低血浆胰岛素水平,增加胰岛素敏感性指数,改善糖耐量,其降糖作用机制之一可能是通过降低血浆胰岛素、增加胰岛素的敏感性。

Effects of Polysaccharide from Atractylodes macrocephala on Blood Glucose and Related Indicators in Spontaneous Type 2 Diabetes db/db Mice

Objective: To observe the hypoglycemia effect of polysaccharide from Atractylodes macrocephala (PAM) in spontaneous type 2 diabetic db/db mice. Method: Type 2 diabetic db/db mice were divided into model group, positive control group (metformin hydrochloride 250 mg · kg^-1), three dose of PAM (300,90,30 mg · kg^-1) and administrated orally once daily for 4 weeks. Body weight and general physical signs were observed during the treatment. The level of fast blood glucose(FBG) was determined at the second and the fourth week and the oral glucose tolerance test (OGTT), fast plasma insulin(Ins) and lipid level were determined after administration for 4 weeks. The insulin sensitivity index (ISI) was calculated based on insulin level. Result: Compared with the model group, db/db mice treated by PAM were in better station, smooth hair, as well as lower food and water intakes. PAM (300,90 mg · kg^-1)could significantly decrease the levels of FBG, Ins, and increase the ISI;meanwhile PAM could decrease the serum glucose levels in 0.5,2 h after glucose administration and lower the area under curve of glucose. Conclusion: PAM could significantly decrease the blood glucose and improve the glucose tolerance. The hypoglycemic effect of PAM may be related to decreasing the plasma insulin and improving the insulin sensitivity.