玉郎伞水提物对慢性鸭乙型肝炎的保护作用

目的:建立鸭乙型肝炎慢性感染模型,研究玉郎伞水提物对鸭慢性乙型肝炎的保护作用。方法:1日龄的广西麻鸭经颈静脉接种0.2 m L的鸭乙型肝炎病毒(DHBV)强阳性血清,7 d后用荧光定量PCR筛选DHBV阳性鸭,随机分为4组,即:模型组,玉郎伞水提物高、低剂量组(按生药量计分别为20,10 g·kg-1)和秋水仙碱组(0.02 g·kg-1)。将未感染鸭作为正常组。除正常组外,其余各组每周均接种0.2 m L的DHBV强阳性血清,直到实验结束,以造成鸭乙型肝炎慢性感染。从造模第25周开始,连续灌胃给药5周。末次给药24 h后,所有动物从颈静脉采血,测定血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)活性,血清鸭乙型肝炎病毒表面抗原(DHBs Ag),DHBV-DNA含量。将所有动物处死,迅速摘取肝组织,测定肝匀浆超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)的活性,丙二醛(MDA)的含量,肝组织中羟脯氨酸(Hyp)的含量。通过HE染色观察肝细胞损伤程度。结果:与正常组比较,模型组血清ALT,AST活性,HBs Ag,DHBV-DNA含量均显著升高(P0.05,P0.01)。肝组织中SOD,GSP-Px活性均显著降低,MDA和Hyp含量则显著升高(P0.05,P0.01)。与模型组比较,玉郎伞水提物高、低剂量组均能降低血清ALT活性和DHBV-DNA含量(P0.05,P0.01),均能增加肝组织匀浆中GSH-Px活性,降低MDA和Hyp的含量(P0.05,P0.01)。玉郎伞水提物高剂量组能降低血清AST活性和HBs Ag含量,增加肝组织SOD活性(P0.05,P0.01)。HE染色显示玉郎伞水提物能显著减轻肝细胞损伤。结论:玉郎伞水提物对DHBV所致的慢性鸭乙型肝炎有一定的保护作用。

Protective Effect of Millettia pulchra Aqueous Extract on Chronic Hepatitis B Duck

Objective: To establish the chronic hepatitis B duck model and study the protective effect of Millettia pulchra aqueous extract on chronic hepatitis B duck. Method: One-day old Guangxi shelducks were infected with 0.2 mL duck hepatitis B virus (DHBV). The infected ducks, detected by real-time PCR 7 days after infection, were divided into model group, M. pulchra high-dose and low-dose group (20,10 g · kg^-1) and colchicine group(0.02 g · kg^-1), and the uninfected ducks were served as normal group. In addition to the normal group, the rest of ducks were vaccinated with 0.2 mL DHBV strong positive serum per week, until the end of the experiment. The corresponding drugs were successively administrated for 5 weeks after 25 weeks of DHBV attack. Blood and liver tissue were collected 24 hours after the last administration. The activity of alanine aminotransferase (ALT) and aspartate amino transferase (AST),and the content of duck hepatitis B virus surface antigen (DHBsAg) and duck hepatitis B virus DNA(DHBV-DNA) in serum were measured. The activity of superoxide dismutase (SOD) and glutathion peroxidase (GSH-Px), and the level of malondialdehyde (MDA) and hydroxyproline (Hyp) in liver homogenate were determined.Hematoxylin-eosin (HE) staining was used to observe the damage degree of liver cells. Result: In model group, the levels of ALT and AST, and the content of DHBsAg and DHBV-DNA in serum were significantly increased;the activity of SOD and GSH-Px was significantly decreased, and the levels of MDA and Hyp were significantly increased, compared with normal group. M. pulchra aqueous extract (20,10 g · kg^-1) significantly reduced the activity of ALT, and the content of DHBV-DNA in serum, increased the activity of GSH-Px, and decreased the levels of MDA and Hyp in liver. M. pulchra aqueous extract (20 g · kg^-1) significantly reduced the activity of AST, increased the activity of SOD. HE staining showed that M. pulchra aqueous extract could significantly decrease the damage degree of liver cells. Conclusion: Millettia pulchra aqueous extract has certain protective effect on chronic hepatitis B duck caused by DHBV.