P16基因缺失与各型白血病关系

Ｐ１６基因缺失在人类不同组织来源的细胞株和原发肿瘤中频繁发生，为探讨Ｐ１６基因缺失与各型白血病的关系，采用ＰＣＲ扩增技术检测了３０例白血病，结果显示：１７例ＡＬＬ中有６例Ｐ１６基因缺失，其中８例Ｔ－ＡＬＬ中有５例、９例Ｂ－ＡＬＬ中有１例Ｐ１６基因缺失，而在５例ＣＭＬ、８例ＡＭＬ中Ｐ１６基因存在。结果表明：Ｐ１６基因缺失与ＡＬＬ尤其是Ｔ－ＡＬＬ有关。Ｐ１６基因检测对ＡＬＬ发病机制的探讨以及诊断都有一定的指导意义

Association of P16 Gene Deletion with Leukemia

Deletions of P16 gene frequently occurred in cell lines of different origin and human primary tumors. To figure out the association of P16 gene deletion with leukemia, we studied 30 cases of primary leukemias including 12 cases of ALL and 8 cases of acute myeloblastic leukemias(AML) and 5 cases of chronic myeloid leukemias(CML) by polymerase chain reaction(PCR), using Molt 4 cell line as positive control and HL 60 cell line as negative control. Our results indicate that six of the seventeen cases of ALL(35%) including five of the eight cases of T cell ALL(62.5%) and one of the nine cases of B cell ALL(11%) exhibited homozygous deletions of P16 gene but no deletion was found in any one of the 8 of AML, 5 of CML. These results demonstrate that the P16 gene homozygous deletion involved in ALL, especially in T ALL. Detection of P16 gene may be useful for the elucidation of pathogenesis of ALL.