Influence of polymer membrane porosity on C3A hepatoblastoma cell adhesive interaction and function |
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Authors: | Krasteva Natalia Seifert Barbara Albrecht Wolfgang Weigel Thomas Schossig Michael Altankov George Groth Thomas |
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Institution: | Institute of Biophysics, Bulgarian Academy of Sciences, Acad G Bonchev Str, Bl 21, 1113 Sofia, Bulgaria. |
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Abstract: | The effect of the porosity of acrylonitrile-N-vinylpyrrolidone copolymer membranes on human C3A hepatoblastoma cell adhesive interaction and functioning is investigated on four membranes with an average pore size ranging between 6 and 12 nm. Adhesion of C3A cells was quantified and characterized by studying overall cell morphology and focal adhesion formation. Cell-cell interactions were characterized by E-cadherin expression and organization. Cell growth, fibronectin synthesis and cytochrome P450 activity were estimated as criteria of functional cell activity. The results suggest that membrane porosity influences the initial cell-surface interactions since an increasing pore size augmented cell adhesion and aggregate formation. Cell growth after 7 d was diminished on membranes with an average pore size of 12 nm. The activity of P450 measured by 7-ethoxycoumarin conversion at day 7 was influenced by membrane topography representing a clear optimum in the range of 7-10 nm pore size. These results indicate that membrane porosity is a determinant for the function of hepatocytes in extracorporal liver assist devices. |
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