硫普罗宁和脾切除对大鼠肝脏缺血再灌注损伤的保护作用

目的:探讨硫普罗宁和脾切除对大鼠肝脏缺血再灌注损伤的保护作用及其抗肝脏缺血再灌注损伤的作用机制。方法:雄性Wistar大鼠90只,随机分成正常对照组、缺血再灌注组、硫普罗宁+无脾切除组(即硫普罗宁组)、脾切除组和硫普罗宁+脾切除组,每组18只。采用无损伤血管夹夹闭供应肝左叶、中叶的肝动脉和门静脉,观察硫普罗宁在有无脾脏条件下肝脏缺血再灌注损伤后血清中丙氨酸氨基转移酶(ALT)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平,并取下肝脏用HE染色判断肝细胞坏死程度及中性粒细胞浸润情况。结果:缺血再灌注组复流后血清中ALT、MDA水平明显高于正常对照组(P<0.01)。而硫普罗宁组和脾切除组血清中ALT、MDA水平明显低于缺血再灌注组(P<0.01)。硫普罗宁+脾切除组血清中ALT、MDA水平明显低于缺血再灌注组、硫普罗宁组和脾切除组(P<0.01)。缺血再灌注组总SOD(T-SOD)活力明显低于正常对照组(P<0.01)。而硫普罗宁组和脾切除组T-SOD活力明显高于缺血再灌注组(P<0.01)。而硫普罗宁+脾切除组T-SOD活力明显高于缺血再灌注组、硫普罗宁组和脾切除组(P<0.01)。HE染色肝脏坏死程度及中性粒细胞检测结果为缺血再灌注组肝脏坏死明显,中性粒细胞明显增多(P<0.01)。硫普罗宁组、脾切除组和硫普罗宁+脾切除组肝脏坏死程度及中性粒细胞记数明显低于缺血再灌注组(P<0.05~P<0.01)。结论:硫普罗宁和脾切除均可提高肝脏缺血再灌注大鼠的SOD活力,降低MDA含量,减轻肝脏缺血再灌注损伤程度。SOD水平比重提高可能更有助于减轻肝脏缺血再灌注损伤。

The protective effect of tiopronin and splenectomy on hepatic ischemia reperfusion injury in rats

Objective：To study the protective effect of tiopronin and splenectomy on the hepatic ischemia reperfusion injury in rats, and to investigate its mechanism. Methods：Ninety male Wistar rats were divided into sham operation group, ischemia-reperfusion（IR） group,tiopronin group, splenectomy group and fiopronin ＋ splenectomy group. The hepatic artery and portal vein to the left and median liver lobes were occluded ; the levels of serum alanine amintransperase （ ALT）, superoxide dismutase （ SOD ）, malondialdehyde （MDA） and the pathological variation were observed; the necrosis of the hepatocyte and the infiltration of neutrophilic granulocytes were determined by HE staining. Results：The levels of ALT and MDA in serum in IR group was significant higher than that in the sham group（P 〈0.01 ） ;the levels of ALT and MDA in tiopronin group and splenectomy group were significantly lower than that in IR group （P 〈 O. 01 ）, and the level of ALT and MDA in tiopronin ＋ splenectomy group were significantly lower than that in IR group, tiopronin group and splenectomy group（ P 〈 0.01 ）. The level of SOD in serum in tiopronin ＋ splenectomy group was significantly higher than that in IR, tiopronin and splenectomy group（ P 〈 O. 01 ）, and the difference was significant between IR group and tiopronin groups（ P 〈 0.01 ） ;histological lesion of the liver was observed in IR group, and there was an obvious increase of neutrophilic granulocytes （P 〈 0.01 ） ;the necrosis of the liver and the number of neutrophilic granulocytes in tiopronin group and splenectomy groups were significantly lower than those in IR group（ P 〈 O. 05 to P 〈 0.01 ）. Conclusions： Tiopronin can increase the activity of SOD, decrease the level of MDA and protect the liver from ischemia reperfusion injury in rats, and splenectomy has the same function as tiopronin. The combined therapy of tiopronin and splenectomy may enhance the protective effect on the hepatic ischemia reperfusion injury in rats.