蛋白激酶C抑制剂对沙土鼠和大鼠实验性脑缺血所致脑水肿的影响

以沙土鼠单侧结扎颈总动脉造成单侧脑水肿病理模型,观察了两种蛋白激酶C(PKC)抑制剂1-(5-异喹啉磺酰基)-2-甲基哌嗪(H-7)及苦参碱(Mat)对实验性脑缺血所致的脑水肿的影响。结果表明:缺血前30 min ip H-7(6.25和12.5mg·kg^-1)可有效减轻沙土鼠单侧结扎颈总动脉7h所致的结扎侧脑半球的水肿程度,同时减少该侧脑半球电解质的蓄积;Mat(25和50mg·kg^-1)对沙土鼠单侧结扎颈总动脉7h以及Sprague-Dawley大鼠中动脉阻塞1h后再灌流24h所致的脑水肿均有防治作用。

EFFECTS OF INHIBITOR OF PROTEIN KINASECON BRAIN EDEMA FORMATION EVOKED BY EXPERIMENTAL CEREBKAL ISCHEMIA IN GERBILS AND RATS

Recent studies have suggested that protein kinase C(PKC)may be involved in thefoimation of brain edema.In this paper,the effects of two kinds of PKC inhibitors,H-7 and matrine,were examined on the brain edema formation in experimental models.The resuIts showed thatpretreatment with H-7 6.25 and l2.5mg·kg^-1 prevented the accumulation of water and certainelectrolytes in the unilateral hemisphere of the brain evoked.by ligation of a single common carotidartery in Mongolian gerbil; pretreatment with matrine 25 and 50 mg·k-1 reduced the extent ofcerebral edema foimation evoked by ligation of a single common carotid artery in gerbil and by middlecerebral artery occlusion in Sprague-Dawley rats.These resuIts present new evidence for theinvolvement of PKC in the formation of brain edema.