黑加仑提取物保护血管内皮细胞氧化损伤的实验研究

目的研究黑加仑提取物对过氧化氢所致血管内皮细胞ECV-304损伤的影响。方法建立体外过氧化氢损伤模型,测定细胞存活率、上清液中丙二醛(MDA)含量、乳酸脱氢酶(LDH)活力、一氧化氮(NO)、内皮素(ET)和前列环素(PGI2)含量,并运用流式细胞仪观察黑加仑提取物对过氧化氢对内皮细胞ECV-304凋亡的影响。结果黑加仑提取物各剂量都能显著提高H2O2损伤内皮细胞的存活率(P<0.05);显著抑制H2O2损伤引起内皮细胞的LDH和ET的释放(P<0.05);显著减少H2O2损伤的内皮细胞MDA的生成(P<0.05);增加H2O2损伤的内皮细胞NO和PGI2水平(P<0.05)。黑加仑提取物的各组、阳性对照组和正常对照组早期凋亡率和总凋亡率明显低于H2O2模型组(P<0.05)。结论黑加仑提取物对过氧化氢损伤的脐静脉内皮细胞ECV-304具有保护作用;黑加仑提取物可降低H2O2诱导的ECV-304凋亡的早期凋亡率和总凋亡率,以达到保护内皮细胞的作用。

Experiment study of blackcurrant on vascular endothelial cells injury induced by hydrogen peroxide

Objective To study the effect of Blackcurrant on human umbilical vein endothelial ECV-304 cells injury induced by hydrogen peroxide(H_2O_2). Methods The H_2O_2 damagect model was established. The effect of Blackcurrant on injury of ECV-304 activity induced by H_2O_2 was determined by MTT assay. The levels of MDA, NO, ET, PGI2and LDHactivity in cell homogenate were measured with corresponding. Observed the influence of black currant extracts apoptosis induced the ECV-304 by H_2O_2 by flow cytometry. Results Blackcurrant could inhibit the hypoxia induced ECV-304 reduction(P < 0.05), decrease LDH activity(P < 0.05), reduce the MDA and ET production(P < 0.05), and increased the contents of nitric oxide (NO)and PGI2 (P < 0.05). The apoptosis rate and total apoptosis rate of blackcurrant extracts groups/positive control group/control group are obvious lower than H_2_O2 model group(P < 0.05). Conclusion The blackcurrant ethyl acetate extract can protect HUVEC damaged by H_2O_2, the mechanism maybe related to antioxidant. And it can decrease the apoptosis rate and total apoptosis rate of ECV-304 apoptosis induced by H_2O_2 in order to protect ECV-304.