| 铅对大鼠脑突触体钙调素的抑制作用 |
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| 引用本文： | 丁训诚,郑晔.铅对大鼠脑突触体钙调素的抑制作用[J].卫生毒理学杂志,1993,7(4):209-211. |
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| 作者姓名： | 丁训诚 郑晔 |
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| 作者单位： | 中国生育调节药物毒理检测中心，中国生育调节药物毒理检测中心，中国生育调节药物毒理检测中心，中国生育调节药物毒理检测中心 上海 200032，上海 200032，上海 200032，上海 200032 |
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| 摘 要： | 用体外法,将大鼠脑突触体膜,在不同浓度的Pb~(2+)作用下分别测定CaM依赖的Ca~(2+)-ATP酶和CaM活性的IC_(50)值。结果表明,Pb~(2+)对Ca~(2+)-ATP酶活性IC_(50)为 1.62mM;对CaM活性IC_(50)为3.85mM。当向不含CaM的脑突触体加入3.0～10.0μg的外源性CaM后,被Pb~(2+)抑制的Ca~(2+)-ATP酶活性均有显著的逆转而恢复或接近正常水平。推测Pb~(2+)主要直接作用在脑突触体膜的CaM,通过CaM分子构象改变,抑制Ca~(2+)-ATP酶,这可能是铅神经毒性的分子基础。
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| 关 键 词： | 铅 调钙蛋白 毒理学 无机毒物 |
Inhibition of lead on calmoduin of rat brain synaptosomes |
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| Ding Xuncheng et al National Evaluation Centre for the Toxicology of Fertility Regulating Druges shanghai.Inhibition of lead on calmoduin of rat brain synaptosomes[J].Journal of Health Toxicology,1993,7(4):209-211. |
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| Authors: | Ding Xuncheng National Evaluation Centre for the Toxicology of Fertility Regulating Druges shanghai |
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| Institution: | Ding Xuncheng et al National Evaluation Centre for the Toxicology of Fertility Regulating Druges shanghai 200032 |
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| Abstract: | In vitro effects of lead on Ca~(2+)-ATPase of rat brain synaptosomes in the absence andpresence of calmoduin (CaM) were studied. The rat synaptic membrances were prepared andCaM was depleted by washing with 1mM EGTA. Pb~(2+). inhibited the basal as well as CaM-stimulated Ca~(2+)-ATPase in a concentration-dependent manner, suggesting the Pb~(2+) interactionwith calcium pump. Further, the inhibition of CaM-stimulated Ca~(2+)-ATPase activity by Pb~(2+)could be reversed by excessive addition of CaM. Exogenous CaM restored the Pb~(2+) inhibitedCa~(2+)-ATPase activity approximate the original level. These results suggest that Pb~(2+) mayalter calmodulin-regalated synaptic processes in the brain and could constitute a possiblemolecular mechanism for the lead nurotoxicity. |
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| Keywords: | Lead Calmodulin Ca~(2+)-ATPase |
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