氟砷染毒大鼠其子代脾脏氧化性损伤的研究

目的：揭示氟砷共存时对子代健康影响。方法：采用两代一窝繁殖实验 的方法，测定Wistar大鼠暴露于氟砷后其子代脾脏中氟砷的蓄积水平、脂质过氧化水平和抗氧化能力，以及病理组织学检查。结果：随着暴露剂量的增加脾脏中氟、砷含量显著增加，SOD和GSH－Px活性随之降低， 而LPO含量却逐渐增加；停止氟砷暴露8周后,脾脏中氟砷含量显著降低，酶的活性有所恢复，而LPO含量明显减少。结论：氟砷联合染毒可导致大鼠子代脾脏的氧化性损伤。

A research on the oxidative damage of spleen of rat's offspring caused by fluoride-arsenic exposure

Objective: To explore the effects of the fluoride arsenic exposure to the health of offspring. Methods: The levels of accumulation and lipid peroxidation and the abilities of antioxidation were determined in spleen of their offspring after wistar rats were exposed to fluoride and arsenic with the method of two generations one nest reproductive test. Results: The contents of fluoride and arsenic in spleen of the offspring correlated with the dose taken by their exposed parents. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH Px) were decreased when increased of exposed dose. In contract, the concentration of lipid peroxides (LPO) was increased. After stopping the exposure for 8 weeks, the contents of fluoride and arsenic in spleen were decreased distinctly; the activities of SOD and GSH Px were increased, the concentration of LPO decreased. Conclusion: The fluoride arsenic exposure could cause the oxidative damage of spleen of rat's offspring.