响应面分析法优化湿法超微粉碎地龙蛋白的提取工艺

目的:优选采用湿法超微粉碎法提取地龙蛋白的工艺.方法:在单因素试验基础上,以浸泡时间、料液比、提取温度、粉碎时间为影响因素,蛋白质得率为响应值,根据中心组合试验设计原理采用四因素三水平的4.7h分析法,对湿法超 微粉碎提取地龙蛋白条件进行优化.结果:湿法超微粉碎提取地龙蛋白的最佳工艺条件为浸泡时间4.7 h,料液比1∶11,提取温度17℃,粉碎时间10 min.在此条件下蛋白质得率3.46％.结论:湿法超微粉碎提取技术适用于地龙蛋白提取.该试验优选提取工艺稳定可行,适于工业化推广.

Optimization of Extraction Technology for Wet Ultrafine Grinding Technology on Earthworm Protein by Response Surface Methodology

Objective:To optimize extraction technology of earthworm protein by wet ultrafine grinding technology.Method: On the basis of single-factor test,extraction technology of wet ultrafine grinding method on earthworm protein was optimized with soaking time,solid-liquid ratio,extraction temperature and grinding time as influencing factors,yield of protein as response value.According to central composite test design principles,4 factors and 3 levels of response surface analysis method was used.Result: Optimum extraction process of earthworm protein by wet ultrafine grinding technology was as follows:soaked 4.7 h,solid-liquid rate 1∶11,extraction temperature 17 ℃,grinding time 10 min.Under these conditions,yield of protein could be up to 3.46%.Conclusion: Wet ultrafine grinding technology was suitable for extraction of earthworm protein.This optimize extraction technology was stable,feasible and suitable for large-scale promotion.