黄药子抗肿瘤活性组分筛选及作用分析

目的 :分析中药薯蓣科植物黄药子可能的抗肿瘤组分及其作用机制。方法 :将黄药子依次用石油醚、乙醇、水系统地提取 ,采用HepA肿瘤细胞株在小鼠进行体内筛选和研究 ,观察不同组分对肿瘤腹水生长及肿瘤细胞的各种变化 ,分析各组分抗肿瘤作用及特征。结果 :石油醚提取物抑制肿瘤腹水生长作用强于乙醇提取物 ,而水提取物有一定的促生长作用。石油醚提取物能显著延长小鼠存活期 ,低降肿瘤腹水生成量和肿瘤细胞存活率 ,用药后肿瘤细胞再次传代 ,肿瘤腹水的形成时间显著延长。结论 :黄药子石油醚提取物具有显著的抗肿瘤活性 ,且抗肿瘤作用与直接的细胞作用有关。

Anticancer effects of various fractions extracted from Dioscorea bulbifera on mice bearing HepA

Objective: To investigate the anti-cancer activities and the possible mechanism of Chinese herb Dioscrea bulbifera.Method: The herb was extracted sequentially with petroleum ether,ethanol and water. The anticancer screen were carried out in vivo with HepA in mice. Result: The inhibitory effects on the formation of ascites volume and HepA cell viability in ascites were found in those extracted fractions except water fraction,the petroleum ether fraction being the strongest. Life span of mice bearing HepA ascites was prolonged after exposed to 100 mg·kg ^-1 petroleum ether fraction and shortened after exposed to water fraction significantly. Besides, abnormal microstructure on HepA cells surface was found and it was supposed to be potential effect against viability of HepA which was convinced with the regeneration of HepA cells from ascites in mice exposed to petroleum ether fraction. Conclusion: Anticancer active compounds are mainly extracted by petroleum ether from hydrophobic constituents of Dioscrea bulbifera and the anticancer effects were related to direct toxicity on tumor cell.