包被酶和偶联抗体脂质体的制备

用磷脂酰胆碱、神经节苷酯、胆固醇以４５∶１０∶４５的克分比浓度制备出大的单层脂质体，在其水相溶液中分别包被０．５，１，２，４ｍｇ的碱性磷酸酶（ＡＫＰ），经或不经ＴｒｉｔｏｎＸ－１００处理后，与ＡＫＰ的底物作用，于４００ｎｍ处测ＯＤ值。结果显示，当ＡＫＰ为１ｍｇ／ｍｌ时，效果最理想（０．７８４±０．０５６和０．１７５±０．０２７）。该脂质体充入氮气后，于４℃中可保存３个月。用过碘酸钠法分别偶联５，１０，１５ｍｇ／ｍｌ的抗ＡＦＰ于脂质体上，发现随着抗ＡＦＰ浓度的增加，偶联的抗ＡＦＰ的比例升高，而结合抗ＡＦＰ的百分比却有下降趋势。

Preparation of Liposomes Containing Enzyme And Coupled Antibody

Large unilamellar liposomes were prepared from phosphatidylcholine, ganglioside, and cholesterol in a 45, 10 and 45 molaration and contained 0.5, 1,2, 4mg/ml alkaline phosphatase (AKP) respectively in their aqueous phase . The realease of entrapped AKP was recorded on OD400nm by the addition of the substrate with liposomes either treated or not treated by Triton X 100. The results were the best (0.784±0.056 and 0.175±0.027) when the concentration of AKP was 1mg/ml. Thee stability could last 3 months when the liposomes were stored at 4℃ under atmosphere of nitrogen. The results of 5,10 and 15mg AFP coupled seperately to liposomes treated by the periodate demonstrated that the greater the concentration of anti AFP was the high the ratio of the anti AFPcoupled to liposome to phosphatide was , and the lower the percentage of bound anti AFP was.