二甲双胍和黄连素配伍复方对糖尿病大鼠神经系统的影响

目的测试二甲双胍和黄连素配伍复方联合治疗对糖尿病大鼠神经系统的影响。方法口服给予链脲佐菌素（STZ）糖尿病模型大鼠以二甲双胍复方（二甲双胍与盐酸黄连素配比为60：130）3个月，测试糖尿病大鼠痛觉、鼠尾神经传导速度及脑内丙二醛（MDA）含量、Na^＋-K^＋-ATP酶活性等指标。结果二甲双胍复方高、中、低（400，200，100mg／kg）剂量均可使糖尿病大鼠脑内MDA含量明显降低（P〈0．01），并使脑内Na^＋-K^＋-ATP酶活性明显升高（P〈0．01），并且各给药组呈良好的量效关系；各给药组大鼠的神经传导速度均较模型组显著增快（P〈0．01），糖尿病大鼠痛觉潜伏期缩短明显（P〈0．01）。结论二甲双胍和盐酸黄连素配伍复方可显著降低脑内MDA含量，升高Na^＋-K^＋～ATP酶活性，同时显著缩短痛觉潜伏期和提高神经传导速度。

Effect of Compatible Compound Metformin and Berberine on Neural System of Diabetic Rats

Objective To observe the influence of the compatible compound metformin and berberine （CCMB） on the neural system of the diabetic rats. Methods Diabetic rats established with injection of strep tozotocin （STZ） were orally administered with the CCMB （metformin ： berberine =60 ： 130） for 3 months. To detect the indexes of the sense of pain, the conductive velocity of caudal nerve, the content of MDA and the activity of enzyme of Na^＋- K^＋- ATP in brain. Results After the diabetic rats were administered with the CCMB, high dose 400 mg/kg, middle dose 200 mg/kg, low dose 100 mg/kg, respectively, the content of MDA in diabetic rats brain was remarkably decreased （ P 〈 0.01） while the activity of enzyme of Na^＋- K^＋- ATP was increased significantly （ P 〈 0. 01）. Different dose treatment groups expressed a good dose-activity relationship. The conductive velocity of caudal nerve in rats was increased （P〈0.01） significantly and the latent period of sense of pain was shortened （P 〈 0.01） significantly in different dose treatment groups than that in model group. Conclusion CCMB may significantly decrease the content of MDA in diabetic rats brain, improve the activity of enzyme of Na^＋-K^＋-ATP, as well as increase the conductive velocity of caudal nerve and shorten the latent period of sense of pain.