Concentrations of lysosomal cysteine proteases are decreased in renal cell carcinoma compared with normal kidney |
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| Authors: | Heidrun Kirschke Tobias Clausen Barbara Göhring Dagmar Günther Edwin Heucke Friedemann Laube Eckhard Löwe Heinz Neef Henriette Papesch Skadi Peinze Gunnar Plehn Udo Rebmann Ari Rinne Roland Rüdrich Ekkehard Weber |
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| Affiliation: | (1) Institute of Physiological Chemistry, Martin-Luther University, D-06097 Halle (Saale), Germany;(2) Clinic for Urology, Martin-Luther University, D-06097 Halle (Saale), Germany;(3) Clinic for Surgery, Martin-Luther University, D-06097 Halle (Saale), Germany;(4) Department of Pathology, University of Tromsø, N-9001 Tromsø, Norway |
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| Abstract: | Renal cell carcinoma contains significantly lower concentrations of the lysosomal cysteine proteases, cathepsins B, C, H, L and S, than does normal kidney, as shown by several methods, such as activity determination, enzyme-linked immunosorbent assay, immunoblotting and immunohistochemistry. The same low levels of enzyme activity and concentration have been determined in renal cell carcinoma metastases in the lung. Our results on the decreased concentration of cysteine peptidases at the protein level would seem to conflict with earlier results on an increased concentration of the cathepsin L mRNA in renal cell carcinoma.Abbreviations ELISA enzyme-linked immunosorbent assay - FCS fetal calf serum - NHMec 7-(4-methyl)coumarylamide - NHNap 2-naphthylamide - Z benzyloxycarbonyl |
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| Keywords: | Lysosomal cysteine proteases Cathepsins Renal cell carcinoma Kidney |
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