| 介绍一种滤纸片快速回收DNA片段的方法 |
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| 引用本文： | 丁晓华,伍欣星,周勇,魏赛男,苏应斌.介绍一种滤纸片快速回收DNA片段的方法[J].武汉大学学报(医学版),1998(1). |
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| 作者姓名： | 丁晓华 伍欣星 周勇 魏赛男 苏应斌 |
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| 作者单位： | Virus Research Institute，Basic Medical College，Hubei Medical University，Wuhan 430071，China |
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| 摘 要： | 采用置于Ｅｐｐｅｎｄｏｒｆ管中的滤纸片，快速离心的方法回收ＤＮＡ片段。实验表明，该方法是一种简便、快速、可靠的回收ＤＮＡ片段的方法，回收率高。回收的ＤＮＡ片段可进一步用于基因工程中克隆连接、酶切和探针标记。
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| 关 键 词： | 脱氧核糖核酸 电泳 脱氧核糖核酸内切酶类 |
Introduction of a Rapid Method of Filters Recovery of DNA Fragment |
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| Abstract: | ? We estabilished a method of recovery of DNA fragment through filters in the Eppendorf tube and centrifugation. It was found that it was a simple, rapid and reliable method. The rate of recovery was over 80 percent. The DNA fragment may be further digested by restriction enzymes, nick translated by DNase Ⅰ and polymerase Ⅰ and linked by T4 DNA ligase. |
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| Keywords: | MeSH DNA electrophoresis endodeoxyribonuclease |
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