| 一种检测可溶性白细胞介素2受体夹心法ELISA的建立 |
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| 引用本文： | 孙忱 商澎. 一种检测可溶性白细胞介素2受体夹心法ELISA的建立[J]. 中国免疫学杂志, 1991, 7(6): 359-361 |
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| 作者姓名： | 孙忱 商澎 |
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| 作者单位： | 第四军医大学免疫学教研室，第四军医大学免疫学教研室，第四军医大学免疫学教研室，第四军医大学免疫学教研室，第四军医大学免疫学教研室，第四军医大学免疫学教研室，第四军医大学免疫学教研室 西安 710032，西安 710032，西安 710032，西安 710032，西安 710032，西安 710032，西安 710032 |
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| 摘 要： | 采用两种识别不同表位的小鼠抗人白细胞介素2受体(IL-2R)单克隆抗体(McAb),在国内首次建立了检测可溶性IL-2R(sIL-2R)的夹心法ELISA,并进行了初步应用。结果显示:本法特异性强、敏感性高、重复性好、操作简便,能检出正常人、某些患者血清中以及细胞培养上清中sIL-2R,可用于基础和临床免疫学研究。
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| 关 键 词： | 单克隆抗体 夹心法ELISA 细胞 |
Establishment of a sandwich ELISA method for measuring soluble IL-2 receptor |
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| SUN Chen,et al.. Establishment of a sandwich ELISA method for measuring soluble IL-2 receptor[J]. Chinese Journal of Immunology, 1991, 7(6): 359-361 |
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| Authors: | SUN Chen et al. |
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| Affiliation: | SUN Chen,et al. Department of Immunology,The Fourth Military Medical Universith,Xi-an 710032 |
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| Abstract: | Two kinds of mouse monoclonal antibodies which recognize distinct epitopes of the IL-2 receptor (a chain) were used to establish a sandwich ELISA for measuring soluble IL-2 receptors. The results indicated that this method is sensitive and specific for detecting soluble IL-2 receptors of cell culture supernatant and serum from both healthy donor and patients with leukemia. Therefore this sandwich ELISA method is useful for basic and clinic immunology research. |
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| Keywords: | Soluble IL- 2 receptor (sIL- 2R) Sandwich ELISA Monoclonal antibody (McAb) |
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