乳腺癌的DNA含量及其与核内雌激素受体的关系

The relation of deoxyribonucleic acid contents and nuclear estrogen receptors in breast cancers

Flow cytometry was applied to the analysis of DNA contents in 59 cases of breast cancer, among which 28 cases were diploidy cancers, and 31 were aneuploid. DNA index of these cases was found to be ranging from 0.45 to 3.35, including S-phase fraction (SPF) from 2.46% to 78.87%, and proliferation index (PI) from 17.06 to 87.04. In 60 cases of breast carcinoma, nuclear estrogen receptors (ERn) were assayed by immunocytochemistry with ER-monoclonal antibody. 29 of them were ERn-, and 31 were ERn+. The DNA contents and ERn levels, both detected in each of 57 cases, were found to be correlated to some extent. In aneuploidy cancer group, there were more ERn- cases than ERn+ cases (P less than 0.05). The mean value of PI was higher in the ERn- group than in the ERn+ group, and the corresponding ERn- cases were more frequent with increase of PI value. Among those cases in which SPF was over 40%, there were more ERn- cases than ERn+ cases. The relations between ERn levels and DNA contents may represent the degree of differentiation of breast cancers and may help to predict prognosis.