| 改良Bouvet法纯化IgM型单克隆抗体 |
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| 引用本文： | 林料明 吴健. 改良Bouvet法纯化IgM型单克隆抗体[J]. 中国免疫学杂志, 1991, 7(4): 209-211 |
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| 作者姓名： | 林料明 吴健 |
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| 作者单位： | 北京市肿瘤防治研究所，北京市肿瘤防治研究所，北京市肿瘤防治研究所，北京市肿瘤防治研究所 北京 100034，北京 100034，北京 100034，北京 100034 |
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| 摘 要： | 利用IgM的优球蛋白特性,对Bouvet法进行了改良。比较了Bouvet法和改良法对两种IgM型McAb腹水的纯化。结果表明,改良法保留了Bouvet法的优点,而荷载量大大高于Bouvet法,获得了良好的抗体纯度和回收率(1.0～1.5mg/ml腹水)。说明此法为一种简单、快速、高效的纯化IgM型单克隆抗体的方法。
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| 关 键 词： | 单克隆抗体 IgM 纯化 改良 |
A modified method for purification of IgM monoclonal antibodies |
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| LIN Ke-ming,et al.. A modified method for purification of IgM monoclonal antibodies[J]. Chinese Journal of Immunology, 1991, 7(4): 209-211 |
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| Authors: | LIN Ke-ming et al. |
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| Affiliation: | LIN Ke-ming,et al. Beijing Institute for Cancer Research,Beijing 100034 |
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| Abstract: | Based on the euglobin characteristic of IgM, a modified method in which euglobin precipitation and Bouvet method were combined was established. Two IgM momoclonal antibodies (McAb) A12 and PC 1 were purified with the modified method in comparasion with Bouvet method. It was shown that the modified method has a simplicity as does Bouvet method. Moreover, the sample loading amount in the modified method was higher than Bouvet method by 5-10 times, and the yield could reach 1.0-1.5mg/ml. Therefore, the modified method could be used as routine to purify IgM McAb with simplicity, high efficience and more yield. |
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| Keywords: | McAb IgM Purification |
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