| 自由基对体外培养兔关节软骨细胞的影响 |
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| 引用本文： | 康勇,周兰华.自由基对体外培养兔关节软骨细胞的影响[J].中华病理学杂志,1991,20(2):100-102. |
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| 作者姓名： | 康勇 周兰华 |
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| 摘 要： |
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| 关 键 词： | 软骨细胞 自由基 关节疾病 |
The effect of free radicals on the rabbit articular chondrocytes in monolayer culture |
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| Y Kang.The effect of free radicals on the rabbit articular chondrocytes in monolayer culture[J].Chinese Journal of Pathology,1991,20(2):100-102. |
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| Authors: | Y Kang |
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| Institution: | Department of Pathology, Xian Medical University. |
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| Abstract: | By using monolayer culture technique, the effect of endogenous free radicals on rabbit articular chondrocytes was studied. The free radicals were provided through the action of xanthine oxidase (XO) on xanthine (X). The amount of DNA in chondrocytes was measured with ethidium bromide method for direct fluorometric estimation of DNA. The synthesis of proteoglycan (PG) was assayed through 35S-Na2SO4 incorporation followed by inverted microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results indicated that chondrocytes could be damaged by endogenous free radicals generated by the action of XO on X, and these free radicals may be related to the pathogenesis of cartilage aging, degeneration, degenerative joint disease and arthritis. |
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