紫薯花青素抗结肠癌作用及其诱导细胞凋亡机制

目的: 探讨紫薯中原花青素(PCI)对人结肠癌细胞(HCT-116)的细胞毒性作用,阐明其诱导细胞凋亡的机制。方法: 取处于对数生长期HCT-116细胞和人成纤维细胞HF-91随机分为对照组(PBS组)、阳性药组25 mg·L^-1顺铂(DDP)]和PCI组(终浓度分别为25、50和100 mg·L^-1)。采用CCK8法检测细胞增殖抑制率,AO/EB法荧光显微镜观察细胞凋亡情况,流式细胞术检测JC-1线粒体膜电位变化,qPCR法检测p53和caspase-3 mRNA表达水平。结果: 与DDP组比较,100 mg·L^-1 PCI组HCT-116细胞增殖抑制率明显升高(P<0.05),各浓度PCI组HF-91细胞增殖抑制率明显降低(P<0.05),且呈浓度依赖性。与DDP组比较,50和100 mg·L^-1 PCI组HCT-116细胞凋亡率明显升高(P<0.05)。与对照组比较,DDP组和不同浓度PCI组HCT-116细胞中caspase-3和p53 mRNA表达水平均明显升高(P<0.05);与DDP组比较,不同浓度 PCI组HCT-116细胞中caspase-3 mRNA表达水平均明显升高(P<0.05),100 mg·L^-1 PCI组HCT-116细胞中p53 mRNA表达水平明显升高(P<0.05),且呈浓度依赖性。结论: 紫薯花青素对消化系统肿瘤细胞具有较强的毒性作用,而对正常细胞毒性较小。

Inhibitory effect of proanthocyanidins on human colorectal carcinoma and its apoptosis-induction mechanisms

Objective To explore the cytotoxicity of anthocyanins from purple sweet potato on the human colorectal carcinoma cells (HCT-116),and to illuminate the mechanism of inducing apoptosis. Methods The HCT-116 cells and fibroblasts HF-91 at the logarithmic phase were divided intocontrol group(PBS),positive drug group (25 mg·L^-1 DDP),PCI groups (final concentrations:25,50,and 100 mg·L^-1). The inhibitroy rates of cell proliferation were detected with CCK8 assay;the apoptosis was observed using AO/EB method;the JC-1 mitochondrial membrane potential was detected by flow cytometry; the expression levels of p53 and caspase-3 mRNA were measured by qPCR method. Results Compared with DDP group,the inhibitory rate of proliferation of HCT-116 cells in 100 mg·L^-1 PCI group was increased(P<0.05),and the inhibitory rates of proliferation of HF-91 cells in different concentraitons of PCI groups were decreased(P<0.05) in a concentration-dependent manner.Compared with DDP group,the apoptotic rates of HCT-116 cells in 50 and 100 mg·L^-1 PCI groups were increased(P<0.05).Compared with control group,the expression levels of p53 and caspase-3 mRNA in HCT-116 cells in DDP group and different concentrations of PCI groups were increased(P<0.05).Compared with DDP group,the expression levels of caspase-3 mRNA in different concentrations of PCI groups were significantly increased(P<0.05),and the expression level of p53 mRNA in 100 mg·L^-1 PCI group was obviously increased(P<0.05). Conclusion Proanthocyanidin has strong cytotoxicity on the digestive system tumor cells,and has little cytotoxicity on the normal cells.