MMP-2和MMP-9在食管鳞状细胞癌患者癌组织中的表达及其意义

目的: 探讨食管鳞状细胞癌患者癌组织中基质金属蛋白酶2(MMP-2)和基质金属蛋白酶9(MMP-9)表达,分析二者的关联性。方法: 选取食管鳞状细胞癌手术离体标本100例,取癌组织和对应的癌旁正常组织,采用逆转录-聚合酶链式反应(RT-PCR)法检测MMP-2和MMP-9 mRNA表达,采用Western blotting法检测MMP-2和MMP-9蛋白表达;采用Pearson相关分析法分析癌组织中MMP-2和MMP-9 mRNA表达的相关性。结果: 食管鳞状细胞癌组织和癌旁正常组织中MMP-2 mRNA阳性表达率分别为89.0%(89/100)和76.0%(76/100),两者比较差异有统计学意义(χ²=5.853,P=0.016);食管鳞状细胞癌组织和癌旁正常组织中MMP-9 mRNA阳性表达率分别为48.0%(48/100)和16.0%(16/100),两者比较差异有统计学意义(χ²=23.529,P=0.000)。MMP-2 mRNA阳性表达率与食管鳞状细胞癌的浸润深度有关联(χ²=4.971,P=0.026),与淋巴结转移无关联(χ²=2.127,P=0.145);MMP-9 mRNA阳性表达率与食管鳞状细胞癌的浸润深度有关联(χ²＝4.421,P=0.035),与淋巴结转移无关联(χ²=0.617,P=0.432)。MMP-2 mRNA与MMP-9 mRNA在食管鳞状细胞癌组织中的阳性表达率呈正相关关系(r=0.274,P=0.006)。结论: MMP-2和MMP-9可能参与了食管鳞状细胞癌的发生发展,但在食管鳞状细胞癌淋巴结转移方面可能不发挥关键作用。

Expressions of MMP-2 and MMP-9 in tumor tissue of patients with esophageal squamous cell carcinoma and their significances

Objective To explore the expressions of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) in the tumor tissue of the patients with esophageal squamous cell carcinoma, and to analyze the correlation between the expressions of MMP-2 and MMP-9. Methods 100 spicemens of surgical removed esophageal squamous cell carcinoma were chosen. The tumor tissue and the adjacent normal esophagel mucosa tissue were selected.RT-PCR method was used to detect the expressions of MMP-2 and MMP-9 mRNA in the esophageal squamous cell carcinoma tissue and the adjacent normal tissue.Western blotting method was used to testify the expressions of MMP-2 and MMP-9 proteins;the correlation between the expressions of MMP-2 and MMP-9 was analyzed by Pearson correlation analysis. Results The positive expression rate of MMP-2 mRNA in carcinoma tissue was 89.0%(89/100), the positive expression rate of MMP-2 mRNA in adjacent normal tissue was 76.0%(76/100),and there was statistical difference between two groups(χ²=5.853,P=0.016).The positive expression rate of MMP-9 mRNA in carcinoma tissue was 48.0%(48/100),the positive expression rate in adjacent normal tissue was 16.0%(16/100),and there was statistical difference of the positive expression rate between two groups(χ²=23.529,P=0.000).There was a positive correlation between the expression of MMP-2 mRNA and the tumor invasion depth(χ²=4.971,P=0.026), but there was no correlation between the expression of MMP-2 mRNA and the lymph node metastasis(χ²=2.127,P=0.145).There was a positive correlation between the expression of MMP-9 mRNA and the tumor invasion depth(χ²=4.421,P=0.035), but there was no correlation between the expression of MMP-9 mRNA and lymph node metastasis(χ²=0.617,P=0.432). There was positive correlationship between the positive expression rates of MMP-2 mRNA and MMP-9 mRNA in esophageal cancer tissue(r=0.274,P=0.006). Conclusion The MMP-2 and MMP-9 may play a very important role in the occurrence and development of esophageal squamous cell carcinoma,however they have no affection on lymph node metastasis of esophageal squamous cell carcinoma.