男性人群中载脂蛋白E基因多态性与血脂水平的关联性分析

目的: 探讨男性人群中载脂蛋白E(APOE)的基因多态性与血脂水平的关联性,为APOE基因作为诊断血脂异常指标提供理论依据。方法: 从3家重庆医院体检中心随机抽取男性356人,其中242例高血脂患者作为病例组,114名健康者作为对照组。检测研究对象静脉血中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)和低密度脂蛋白(LDL-C)水平,并测量其身高和体质量。采用基因芯片测序方法对APOE基因进行分型。结果: APOE基因型在人群中分布,共检测出6种基因型,其中E3/3频数最高,为69.10%;APOE等位基因中E2频数为17.98%,E3频数为75.84%,E4频数为6.18%。在病例组中,不同APOE基因型高血脂患者TC水平比较差异有统计学意义(P<0.05),且APOE4基因携带者的TC水平明显高于APOE2和APOE3基因携带者(P<0.05)。多元线性回归分析,APOE多态性与TC、TG呈正相关关系(r=0.157,P<0.05;r=0.276,P<0.05),与HDL水平呈负相关关系(r=-0.153,P<0.01)。结论: 中国男性人群中APOE基因多态性对血脂水平有一定的影响,APOE4基因型携带者TC和TG水平有升高的趋势。

Analysis on association between polymorphism of apolipoprotein E genotypes and blood lipid levels in male population

Objective To investigate the relationships between apolipoprotein E(APOE) genotypes and blood lipid levels in the male population,and to provide theoretical basis for the diagnosis of dyslipidemia indexes. Methods A cross-sectional health survey was carried out in three hospitals in Chongqing.The random samples came from 365 males in physical examination centers;among them 242 patients with high level of blood lipids were used as case group;and the other 114 healthy subjects were used as control group.The plasma levels of total cholesterol (TC),triglycerides(TG),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol(LDL-C) were determined;the heights and body weights were detected.The genotypes of APOE were evaluated by gene chip sequencing method. Results Six kinds of genotypes in the population were find,among them E3/3 had the highest frequency of APOE genotypes (69.10%),meanwhile the frequency of APOE E2 allele was 17.93%.The frequency of E3 was 75.84%,and the frequency of E4 was 6.18%.There were statistically significant differences of the TC levels between the patients with different APOE genotypes in case group (P<0.05),meanwhile the E4 carriers had a higher TC level than E2 and E3 carriers (P<0.05).The multiple linear regression analysis showed that APOE polymorphism was positively related with the levels of TC and TG(r=0.157,P<0.05;r=0.276,P<0.05),however it was negatively related with the HDL level(r=-0.153,P<0.05). Conclusion The polymorphism of APOE is related to the blood lipid levels in Chinese male population,while the patients with APOE4 genotype have higher TC and TG levels.