弓形虫的酶细胞化学及蒿甲醚对其影响的研究

小鼠腹腔接种RH株弓形虫速殖子1万个，2h后分别灌服5％淀粉溶液及蒿甲醚200mg／kg，连续8d，取腹腔液沉淀作电镜酶细胞化学。测定弓形虫虫体胞嘧啶单核苷酸酶（CMP酶）和葡萄糖-6-磷酸酶（G－6－P酶）的定位及用药后2种酶超微结构水平上的变化。结果表明，CMP酶定位于虫体的溶酶体，药物对其无明显影响。G－6－P酶定位于虫体的质膜外及围虫泡内，用药后酶反应减弱，说明药物对该酶的活性有一定影响，这可能是药物抗弓形虫的作用机制之一。

STUDIES ON THE ENZYME CYTOCHEMISTRY OF TOXOPLASMA GONDII AND THE INFLUENCE OF ARTEMETHER

Male mice of Kunmlng strain were infected with 10 000 tachyzoites intraperitoneally. 2 hours after infection the mice were divided into 2 groups and administered 5 % of amylurn and 200 mg/kg of artemether by gavage for 8 consecutive days. The ultrastructural enzyme cytochemical studles on cytidine monophosphatase (CMP ase) and glucose-6-phosphatase (G-6-P-ase) of the parasites were carried out. CMP-ase was found scattered in the lysosomes of the parasites as well as in the macrophages. No differences were observed in the localization and intensity of CMP-ase activity between the nontreated and treated with drug parasites. G6-P-ase was found surrounding the parasite membrane and scattered in the parasitophorous vacuole in the nontreated parasites. After treatment with artemether, the intensity of G-6-P-ase activity was decreased compared with nontreated control parasites. It is suggested that artemether may exert some action on the G-6-Patase of T. gondii and thus influence the energy metabolism of the parasite.