Genotoxicity studies on the root extract of Polygala tenuifolia Willdenow |
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| Affiliation: | 1. Research & Development Center, Braintropia Co. Ltd., Anyang-si, Gyeonggi-do 431-716, Republic of Korea;2. Department of Microbiology, College of Natural Science, Dankook University, Cheonan-si, Chungnam 330-714, Republic of Korea;1. Limnological Institute of the Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya Str., P.O. Box 278, Irkutsk, 664033, Russia;2. Institute of Problems of Chemical Physics of the Russian Academy of Sciences, Academician Semenov Avenue 1, 142432, Chernogolovka, Moscow Region, Russia;3. A.P. Vinogradov Institute of Geochemistry of the Siberian Branch of the Russian Academy of Sciences, 1a Favorsky Str., Irkutsk, 664033, Russia;1. The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310009, China;2. Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, Medical College of Zhejiang University, Hangzhou 310058, China;3. Laboratory Animal Center of Zhejiang University, Hangzhou 310058, China;1. C.A.R., Cambridge, UK;2. Hammersmith Hospital, London, UK;3. Breakspear Medical Group, Hemel Hempstead, Hertfordshire, UK;1. Faculty of Aerospace Engineering, University Politehnica of Bucharest, Splaiul Independentei 313, RO-060042, Bucharest, Romania;2. CORIA - UMR6614, Université et INSA de Rouen, Campus du Madrillet - BP 8, 76801 Saint Etienne duRouvray Cedex, France;1. Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China;2. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;3. Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu 611130, China;4. Animal Nutrition Institute, Sichuan Academy of Animal Science, Chengdu 610066, China;5. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China |
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| Abstract: | The root of Polygala tenuifolia Willdenow has been used for the treatment against insomnia, amnesia, depression, palpitations with anxiety, and memory improvement. However, there is no sufficient background information on toxicological evaluation of the root to given an assurance of safety for developing dietary supplements and functional foods. As part of a safety evaluation, the potential genotoxicity of the root extract of P. tenuifolia was evaluated using a standard battery of tests (bacterial reverse mutation assay, chromosomal aberrations assay, and mouse micronucleus assay). In a reverse mutation assay using four Salmonella typhimurium strains and Escherichia coli, the extract did not increase the number of revertant colonies in any tester strain with or without metabolic activation by S9 mix, and did not cause chromosomal aberration in short-period test with the S9 mix or in the continuous (24 h) test. A bone marrow micronucleus test in ICR mice dosed by oral gavage at doses up to 2000 mg/kg/day showed no significant or dose dependent increase in the frequency of micronucleated polychromatic erythrocytes (PCE). These results indicate that ingesting the rot extract P. tenuifolia is not genotoxic at the proper dose. |
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| Keywords: | Genotoxicity Reverse mutation Chromosomal aberration Micronucleus test |
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