Mutagenicity and chemopreventive activities of Astronium species assessed by Ames test |
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| Affiliation: | 1. Department of Biological Sciences and Health, Centro Universitário de Araraquara, UNIARA, CEP 14801-340 Araraquara, São Paulo, Brazil;2. Department of Biological Sciences, Faculty of Pharmaceutical Sciences of Araraquara, UNESP-São Paulo State University, CEP 14801-902 Araraquara, São Paulo, Brazil;3. Department of Organic Chemistry, Chemistry Institute of Araraquara, UNESP-São Paulo State University, CEP 14801-970 Araraquara, São Paulo, Brazil;4. UNESP-São Paulo State University, Experimental Campus of the Paulista Coast, CEP 11330-900 São Vicente, São Paulo, Brazil;1. Laboratório de Imunofarmacologia, Departmento de Fisiologia e Patologia, Universidade Federal da Paraíba, João Pessoa, PB, Brazil;2. Faculdade São Francisco da Paraíba, Cajazeiras, PB, Brazil;3. Laboratório de Imunologia Viral, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil;4. Laboratório de Fitoquímica, Departmento de Ciências Farmacêuticas, Universidade Federal da Paraíba, João Pessoa, PB, Brazil;1. Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, School of Pharmacy, Shaanxi College of Traditional Chinese Medicine, Xianyang 712046, China;2. Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Collaborative Innovation Center for Chinese Medicine in Qinba Mountains, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, China;3. The First Hospital of Xi’an, Xi’an 710002, China;1. Department of Pharmaceutical Sciences, CCS, Universidade Federal de Santa Catarina – UFSC, Florianópolis, SC, Brazil;2. Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Greece;3. Laboratory of Neurobiology of Pain and Inflammation, Department of Physiological Sciences, CCB, Universidade Federal de Santa Catarina – UFSC, Florianópolis, SC, Brazil;1. Programa de Pós-graduação em Biologia Experimental, Universidade Federal de Rondônia, Porto Velho, RO, Brazil;2. Colégio de Aplicação, Universidade Federal do Acre, Rio Branco, AC, Brazil;3. Laboratório de Química de Produtos Naturais, Universidade Federal de Rondônia, Porto Velho, RO, Brazil;4. Programa de Pós-graduação em Biodiversidade e Biotecnologia da Amazônia Legal (Rede Bionorte), Manaus, AM, Brazil;5. Programa de Pós-graduação em Biologia Celular e Molecular Aplicada à Saúde, Universidade Luterana do Brasil, Canoas, RS, Brazil;6. Centro Universitário Luterano de Ji-Paraná, Ji-Paraná, RO, Brazil;7. Departamento de Ciências Biológica, Universidade Federal de Rondônia, Porto Velho, RO, Brazil;8. Departamento de Química, Universidade Federal de Rondônia, Porto Velho, RO, Brazil;1. Laboratório de Pesquisa em Fármacos, Departamento de Ciências Biológicas e Saúde, Universidade Federal do Amapá (UNIFAP), Rodovia Juscelino Kubitschek, S/N, Campus Marco Zero, Macapá, AP CEP 68903-419, Brazil;2. Laboratório de Modelagem e Química Computacional (LMQC), Departamento de Ciências Biológicas e Saúde, Universidade Federal do Amapá (UNIFAP), Rodovia Juscelino Kubitschek, S/N, Campus Marco Zero, Macapá, AP CEP 68903-419, Brazil;3. Programa de Pós-Graduação em Inovação Farmacêutica, Departamento de Ciências Biológicas e Saúde, Universidade Federal do Amapá (UNIFAP), Rodovia Juscelino Kubitschek, S/N, Campus Marco Zero, Macapá, AP CEP 68903-419, Brazil;4. Grupo de Bioinformatica, Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (USP/RP), Avenida Bandeirantes 3900, Monte Alegre, Ribeirao Preto, Sao Paulo CEP 14049-900, Brazil;5. Laboratorio de Farmacología de Productos Naturales, Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México, Ciudad de México, Mexico |
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| Abstract: | In the neotropical savannah, Astronium species are used in popular medicine to treat allergies, inflammation, diarrhea and ulcers. Given that natural products are promising starting points for the discovery of novel potentially therapeutic agents, the aim of the present study was to investigate the mutagenic and antimutagenic activities of hydroalcoholic extracts of Astronium spp. The mutagenicity was determined by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102. The antimutagenicity was tested against the direct-acting and indirect-acting mutagens. The results showed that none of the extracts induce any increase in the number of revertants, demonstrating the absence of mutagenic activity. On the other hand, the results on the antimutagenic potential showed a moderate inhibitory effect against NPD and a strong protective effect against B[a]P and AFB1. This study highlights the importance of screening species of Astronium for new medicinal compounds. The promising results obtained open up new avenues for further study and provide a better understanding the mechanisms by which these species act in protecting DNA from damage. However, further pharmacological and toxicological investigations of crude extracts of Astronium spp., as well as of its secondary metabolites, are necessary to determine the mechanism(s) of action to guarantee their safer and more effective application to human health. |
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| Keywords: | Mutagenicity Antimutagenicity Ames test |
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