年龄对血清皮质醇和硫酸脱氢表雄酮水平及其昼夜变化的影响

目的　研究年龄对男性血清皮质醇和硫酸脱氢表雄酮 (DHEAS)水平及其昼夜节律的影响。方法　 2 6名男性健康志愿者按年龄 (每增龄 10岁 )分为四组 :3 0～ (8名 )、40～ (6名 )、5 0～ (5名 )和 60～(7名 )岁组。 8:0 0～ 2 2 :0 0每 2小时 ,2 2 :0 0～ 8:0 0每小时采血一次 ,ELISA检测血清皮质醇 ,化学发光免疫法检测血清DHEAS。结果　血清皮质醇和DHEAS水平均呈明显昼夜变化 ,余弦法证实有统计学意义。皮质醇峰值 4:0 0～ 6:0 0 ,谷值 18:0 0～ 2 4:0 0 ,较白种人的峰、谷时间提前约 2h ;DHEAS峰值在 16:0 0左右。年龄对血清皮质醇水平及其昼夜变化无明显影响 ,但影响血清DHEAS水平 ,60岁组的血清DHEAS水平较其它 3组明显降低 (P <0 .0 5或P <0 .0 1) ,皮质醇 /DHEAS比值也均显著增高 (均P <0 .0 1)。结论　年龄对血清皮质醇水平及其昼夜变化无明显作用 ,但明显影响血清DHEAS水平。

Effects of age on serum cortisol and dehydroepiandrosterone sulfate levels and their circadian variations

Objective To investigate the effects of age on serum cortisol and dehydroepiandrosterone sulfate (DHEAS) levels and their circadian variations. Methods Twenty-six Chinese healthy male volunteers aged 31-63 years were stratified into 10-year age groups (30 s, 40 s, 50 s and 60 s). Serum cortisol and DHEAS levels were examined on a circadian basis (at 2 h intervals from 8:00 to 22:00 and hourly from 22:00 to 8:00) by ELISA and chemiluminescent immunoassay methods, respectively. Results A statistically significant circadian rhythmicity of both steroids was validated in all age groups. The circadian pattern of serum cortisol was characterized by peak (4:00-6:00) and trough (18:00-24:00), being about 2 h earlier than those usually reported in Caucasians. The peak of DHEAS was around 16:00. In this study, aging had no significant influence on serum cortisol level and its circadian rhythm, but serum DHEAS levels in 60 s group were significantly lower than those in young groups (30 s, 40 s and 50 s) (P<0.05 or P<0.01), and the 60 s group had a significantly higher cortisol/DHEAS molar ratio (all P<0.01) than those in young groups as well. Conclusion Aging does not significantly influence serum cortisol level and its circadian variation, but does have significant effect on serum DHEAS level.