HPLC法测定复方益肝灵片中水飞蓟宾的含量 |
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引用本文: | 古海锋,张小茜,常增荣,傅欣彤,郭洪祝.HPLC法测定复方益肝灵片中水飞蓟宾的含量[J].中国中医药信息杂志,2008,15(7):51-52. |
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作者姓名: | 古海锋 张小茜 常增荣 傅欣彤 郭洪祝 |
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作者单位: | 北京市药品检验所,北京,100035 |
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摘 要: | 目的建立复方益肝灵片中水飞蓟宾的含量测定方法。方法采用HPLC法;流动相:甲醇-水-冰醋酸(48:52:1);检测波长:286nm。结果水飞蓟宾在0.2380~2.380μg范围内线性关系良好,其回归方程为:Y=2.146×10^6X+1.818×10^3,r=0.9999,平均加样回收率为98.17%,RSD=1.3%。结论该方法简单、准确、重复性好,可作为复方益肝灵片的质量控制方法。
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关 键 词: | 高效液相色谱法 复方益肝灵片 水飞蓟宾 |
Determination of Silybin in Fufang Yiganling Tablet by HPLC |
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Institution: | GU Hai-feng, ZHANG Xiao-qian, CHANG Zeng-rong, et al (Beijing Institute for Drug Control, Beijing 100035, China) |
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Abstract: | Objective To establish a method for the determination of silybin in Fufang Yiganling tablet. Methods RP-HPLC was used and the mobile phase was consisted of methanol-water-glacial acetic acid (48 : 52 : 1). The detection wavelength was 286 nm. Results The calibration curve of Silybin was linear in the range of 0.238 0-2.380 μg, linear equation: Y=2.146×10^6X+1.818×10^3, r=0.9999, with average recovery of 98.17% (RSD=1.3%). Conclusions The method is simple, accurate and with good reproducibility. It can be used to control the quality of silybin in Fufang Yiganling tablet. |
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Keywords: | HPLC Fufang Yiganling tablet Silybin |
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