固相微萃取和气相色谱-质谱联用分析罗汉果浸膏的挥发性成分

目的　对罗汉果浸膏的挥发性成分进行分离分析鉴定。方法　采用固相微萃取和气相色谱 -质谱联用技术 ,在固相微萃取实验中使用 85μm聚丙烯酸酯作为萃取纤维 ,并对萃取时间、萃取温度、离子强度、解吸时间等实验条件进行优化 ,应用峰面积归一化法测定各组分的相对含量。结果　在 HP-5MS柱共鉴定出 3 8种化合物 ,主要成分为β-大马酮、β-二氢大马酮、糠醛、苯甲酸苄酯等。结论　本方法为罗汉果资源的合理使用提供了科学依据

Analysis of volatile components in the extract of Siraitia grosvenorii by combination of solid phase microextraction and gas chromatography-mass spectrometry

ObjectiveTo analyze the volatile components in the e xtract of Siraitia grosvenorii (Swingle)C.Jeffrey.MethodsThe volatile components were analyzed by gas chromatography-mass spectrometry(G C-MS) and solid phase microextraction (SPME). In SPME experiments, 85 μm poly a crylamide(PA) fiber was used, the factors affecting quantitative results, includ ing extraction time, extraction temperature, ionic intensity and desorption time were optimized,and their relative contents were determined by area normaliza tion. ResultsThirty-eight kinds of compounds were identified in HP-5MS column , which included Megastigmatrieone,2 -Buten-1-one,1-(2,6,6-trimethyl-1,3-cyclohexadien-1-yl)-,3-Buten-1 -one,4-(2,6,6-trimethyl-1(or2)-cyclohexen-1-yl)-, Furfural, Benzyl Benzo ate,etc.ConclusionScientific data can be provided for exploit ation and appropriate use of Siraitia grosvenorii (Swingle)C.Jeffrey resources.