| 裙带菜多糖诱导人肝癌HepG-2细胞凋亡的研究 |
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| 引用本文： | 邹向阳,王雪,王华新,郭莲英,邢嵘,侯林.裙带菜多糖诱导人肝癌HepG-2细胞凋亡的研究[J].营养学报,2007,29(5):470-472. |
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| 作者姓名： | 邹向阳 王雪 王华新 郭莲英 邢嵘 侯林 |
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| 作者单位： | 1. 大连医科大学基础医学院,大连,116027
2. 辽宁师范大学生命科学学院,大连,116029 |
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| 摘 要： | 目的:检测裙带菜多糖(UPPS)对人肝癌HepG-2细胞生长的抑制作用,并探讨其诱导细胞凋亡和抗肿瘤作用的关系。方法:采用MTT法、流式细胞术检测UPPS对HepG-2细胞生长的抑制作用和诱导细胞凋亡的作用;采用免疫细胞化学染色,观察Bax和Bcl-2表达水平的变化。结果:UPPS能抑制HepG-2细胞增殖,明显高于对照组(P<0.01)。诱导细胞凋亡作用发生在UPPS处理24～48h;UPPS处理HepG-2细胞后,Bax蛋白表达增强,Bcl-2蛋白表达下调。结论:UPPS对HepG-2的抑制作用是通过诱导细胞凋亡实现的,可能与Bcl-2和Bax表达水平的变化有关。
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| 关 键 词： | 裙带菜多糖 HepG-2人肝癌细胞株 细胞凋亡 |
| 文章编号： | 0512-7955（2007）05-0470-03 |
| 修稿时间： | 2006-10-28 |
PROLIFERATION INHIBITION AND APOPTOSIS INDUCTION ON HUMAN HEPATO-CARCINOMA HepG-2 CELLS BY UNDARIA PINNATIFIDA POLYSACCARIDES |
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| ZOU Xiang-yang,WANG Xue,WANG Hua-xin,GUO Lian-ying,XING Rong,HOU Lin.PROLIFERATION INHIBITION AND APOPTOSIS INDUCTION ON HUMAN HEPATO-CARCINOMA HepG-2 CELLS BY UNDARIA PINNATIFIDA POLYSACCARIDES[J].Acta Nutrimenta Sinica,2007,29(5):470-472. |
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| Authors: | ZOU Xiang-yang WANG Xue WANG Hua-xin GUO Lian-ying XING Rong HOU Lin |
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| Institution: | 1.School of Basic Medicine, Dalian Medical University, Dalian 116027; 2. College of Life Science ,Liao Ning Normal University,Dalian 116029, China |
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| Abstract: | Objective: To investigate the inhibitory effect of Undaria pinnatifida polysaccharides (UPPS) on hepatocellular carcinoma HepG-2 cells and its possible mechanism. Method: The effect of inhibiting proliferation and inducting apoptosis of UPPS were determined by means of MTT and FCM. The expression of Bcl-2 and Bax proteins was immunohis to chemcally evaluated after treatment of UPPS. Results: UPPS inhibited HepG-2 cells growth in vitro , significantly higher than the negative control group (P< 0.01), and induced apoptosis after UPPS treatment 24～48h, with decreased expression of Bcl-2 and increased expression of Bax. Conclusion: UPPS inhibits the proliferation of HepG-2 cells by apoptosis, perhaps associated with the change of expression of Bax and Bcl-2. |
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| Keywords: | Undaria pinnatifida polysaccharides (UPPS) hepatocellular carcinoma HepG-2 cells apoptosis |
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