广藿香青枯病菌培养特性的研究

目的 对青枯菌的培养特性进行研究,为更有效地防治广藿香青枯病提供病原菌基础生物学方面的参考.方法 通过测定悬浮培养菌液的吸光度A600,考察青枯菌的生长状态、温度和pH值对青枯菌生长的影响,以及菌体对不同碳源和氮源的利用情况.结果 青枯菌的最适生长温度范围为33～35℃,生长的最适初始pH值为6.5;菌株能利用9种供试...

Cultural characteristics of pathogenic Ralstonia solanacearum from Pogostemon cablin(Blanco)Benth

Objective To study on the cultural characteristics of Ralstonia solanacearum from Pogostemon cablin（Blanco） Benth , which might contribute to the effective control and prevention over the disease. Methods The bacterial growth was determined by A600 of bacterial suspension. Effects of temperature and pH on the multiplication of R. solanacearum, as well as the utilization of carbon and nitrogen nutrient sources were investigated. Results The optimum temperature and pH for bacterial growth was 33 - 35 ℃ and 6.5, respectively. R. solanacearum preferred fructose, maltose, sucrose and sorbitolum among the 9 tested carbon sources to glucose. Organic nitrogens were more favorable for R. solanacearum than the inorangic ones, of which Yeast extract promoted bacterial proliferation remarkably while KNO3 was suppressive. Conclusion The cultural characteristics of R. solanacearum were described, which served for the prevention of R. solanacearum in P. cablin.