木蹄层孔菌多糖对免疫抑制小鼠免疫功能及细胞因子产生的影响

目的 探讨木蹄层孔菌多糖(Fomes fomentarius polysaceharides,FFP,木蹄多糖)对免疫抑制小鼠单核/巨噬细胞吞噬功能、体液免疫功能及T淋巴细胞分泌白介素2(IL-2)、干扰素γ(IFN-γ)的影响.方法 环磷酰胺(15 mg/kg)皮下注射,连续7d,建立免疫抑制小鼠模型,碳粒廓清法检测...

Effects of Fomes fomentarius polysaccharides on immune function and cytokines production of immunosuppressive mice

Objective To investigate the effects of Fomes fomentarius polysaccharides （FFP） on humoral immune response,phagocytic activities of mononuclear macrophages and production of interleukin-2 （IL- 2） and interferon-γ（IFN-5,） by T lymphocytes in immunosuppressive mice. Methods Immunosuppressive mice model were established by subcutaneous administration of cyclophosphamide （15 mg/kg ） for 7 consecutive days. Phagocytic activity of mononuclear macrophages was determined with carbon clearance test. Humoral immunity was evaluated with the production of specific antibody （haemolysin） against rabbit red blood cells （RRBC）. Peripheral white blood ceils was counted under microscope. Cytokines production of T lymphocytes was induced by concanavalin A （Con A）. The amounts of IL-2 and IFN-＇y were measured with ELISA assay. Results It was found that FFP （200 and 100 mg/kg）significantly enhanced the phagocytic activity of mononuclear macrophages, increased the level of serum hemolysin induced by RRBC immunization, raised the total number of peripheral white blood cells and promoted the production of IL-2 and IFN-γ by T lymphocytes in immunosuppressive mice. Conclusion FFP can enhance non-specific immune function and humoral immune response of immunosuppressive mice, and promote production of IL-2 and IFN-γ by T lymphocytes in the mice.