大鼠糖尿病模型早期病理观察指标的筛选

目的 通过腹腔注射链脲佐菌素(STZ)制作大鼠糖尿病模型,筛选成模早期对糖尿病变化敏感的器官组织及生化指标.方法 60只雄性SD大鼠随机分成5组,分别腹腔一次性注射0、20、30、50和80mg/kg的STZ,注射STZ后第2天开始检测各组大鼠的空腹血糖、随机血糖及体质量,第9天实验结束时,取肾脏、胰腺、眼球、心脏、肝...

Selection of pathogenic indicators in the early stage of diabetes rats

Objective To select the sensitive pathogenic and biochemical indicators in the early stage of diabetes rats, diabetes in SD rats was induced by intraperitoneal injection of Streptozotocin at different doses. Methods 60 SD rats were randomly divided into 6 groups, intraperitoneally injected with streptozotocin at the doses of 0, 20,30,50, and 80mg/kg respectively. Fasting blood glucose level, random blood sugar level, and body weight were recorded daily from the second day of injection, pathological examinations of kidney, pancreas, eye ball, liver,spleen,lung,aorta,and testicles were conducted,and serum insulin levels and C-peptide were assayed in the 9th day after the beginning of injection. Results No significant difference of the pathogenic and biochemical indicators of rats from groups intraperitoneally injected with streptozotocin at 20 mg and 30 mg was observed ; Fasting blood glucose level in the groups intraperitoneally injected with streptozotocin at 50 and 80 mg/kg increased significantly, onsets of lesions in kidney, eyeball, and pancreas in the rats were observed, and the number of islets decreased significantly （ P 〈 0.05 ）. And serum insulinand C-peptide levels in the group of rats intraperitoneally injected with streptozotocin at 50 and 80 mg/kg also decreased significantly （ P 〈 0. 05 ）. Conclusion In the early stage of diabetes mellitus, levels of fasting blood glucose, insulin, C-peptide, body- weight,pancreas, kidney and eyes were key monitoring indicators. And no obvious changes of heart, liver, spleen, lung, aorta, and testicles in the rats in the early stage of diabetes were observed.