音乐电针和脉冲电针对慢性应激抑郁模型大鼠不同脑区单胺类神经递质表达的影响

目的：对比观察不同电针对慢性应激抑郁模型大鼠额叶和海马5-HT、DA和NE表达的影响，进一步研究音乐电针与脉冲电针干预治疗抑郁症的疗效差异以及其生物学机制。方法：雄性SD大鼠，随机分为5组：正常对照组、模型组、百忧解组、音乐电针组、脉冲电针组，每组10只。除正常对照组外，其余均采用慢性应激结合孤养的方式造模。采用开野实验、糖水消耗实验和体重检测进行各组的行为学评价；采用放射免疫法分别检测大鼠额叶和海马5-HT、DA和NE的表达。结果：实验第21天后，模型组大鼠行为学指标较正常对照组有明显降低（P＜0．01）；百忧解组、音乐电针组和脉冲电针组与模型组比较，大鼠活动度、糖水消耗量和体重明显升高（P＜0．05）。与正常对照组比较，模型组大鼠额叶和海马组织5-HT、DA和NE的表达量明显降低（P＜0．01）；与模型组相比，百忧解组、脉冲电针组和音乐电针组能提高额叶和海马组织的5-HT、DA、NE的表达量（P＜0．05）；与脉冲电针组比较，音乐电针组海马和额叶组织的5-HT的表达量有显著性差异（P＜0．05）。结论：百忧解、脉冲电针和音乐电针均可改善慢性应激抑郁大鼠的行为学症状，具有一定的抗抑郁作用；音乐电针在提高慢性应激模型大鼠中枢单胺类的神经水平方面优于百忧解和脉冲电针，这可能是音乐电针发挥抗抑郁治疗作用的途径之一。

Influence of Music Electro-acupuncture and Pulsed Electro-acupuncture on the Different Encephalic Regions of Monoamine Neurotransmitter Chronic Unpredictable Mild Stress Depression Model Rats

Objective：To observe the influence of different electro -acupuncture on frontal lobe and hippocampus to 5-HT,DA,NE neurotransmitter ’ s expression of chronic unpredictable mild stress depression model rats , to probe into the mechanism for treating depression .Methods：Fifty male SD rats were randomly divided into five groups：Nor-mal control group, Model group, Fluoxetine group, music electro-acupuncture group and pulsed electro-acupunc-ture group .All groups were treated with the chronic unpredictable mild stress stimulation combined with lonely rai-sing for 21 days except the normal control group .Behavior changes of rats were detected through open -field test , sugar consumption test and body weight examining .Detect the 5-HT,DA,NE of frontal lobe and hippocampal of each rat by radioimmunoassay .Results：After 21 days, Compared with normal control group ,the scores of behavior in model group decreased significantly （P〈0.01）.Compared with model group,the scores of horizontal activity and ver-tical activity,sugar consumption and weight in Fluoxetine group , music electro-acupuncture and pulsed electro -ac-upuncture increased significantly（P〈0.05）.Compared with normal control group ,the contents of frontal lobe and hippocampus’ 5-HT,DA,NE decreased significantly in model group （P〈0.01）.Compared with model group,the contents of frontal lobe and hippocampus ’ 5 -HT, DA, NE increased significantly in Fluoxetine group , music electro-acupuncture and pulsed electro-acupuncture group（P〈0.05）.Compared with pulsed electro-acupuncture group, the contents of 5-HT increased significantly in music electro -acupuncture group（P〈0.05）.Conclusion：Fluoxetine, pulsed electro-acupuncture and music electro-acupuncture could have a certain antidepressant effect on improving the behavior of depressive rats;music electro-acupuncture ’ s regulative effect on monoamine neuro-transmitter is much better than Fluoxetine and pulsed electro -acupuncture, which may be one of the mechanisms for treating depression .