续断壮骨胶囊中总皂苷和川续断皂苷VI含量测定 |
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引用本文: | 章彩霞,金灵华.续断壮骨胶囊中总皂苷和川续断皂苷VI含量测定[J].中国执业药师,2014(2):8-11,15. |
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作者姓名: | 章彩霞 金灵华 |
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作者单位: | 浙江迪耳药业有限公司,浙江 金华321016 |
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基金项目: | 基金项目:国家重大科技专项“创新药物及中药现代化”(2003AA6Z3603) |
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摘 要: | 目的:建立续断壮骨胶囊中总皂苷和川续断皂苷遇的含量测定方法。方法:采用高氯酸显色,紫外-可见分光光度法测定总皂苷含量。采用高效液相色谱法测定川续断皂苷遇含量,Agilent C18柱(250 mm 215;4.6 mm,5μm),乙腈-0.15%三氟乙酸水溶液(29∶71)为流动相,检测波长215 nm ,流量1.0 mL/min。结果:总皂苷平均含量为86.3%,平均加样回收率为99.88%, RSD =1.52%;川续断皂苷遇在64~320μg/mL 范围内呈良好线性关系(r =0.9995),平均加样回收率为100.4%, RSD =0.77%。结论:所建方法准确,能快速测定续断壮骨胶囊总皂苷和川续断皂苷遇的含量,可用于该制剂的质量控制。
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关 键 词: | 续断壮骨胶囊 川续断皂苷遇 高效液相色谱法 含量测定 AsperosaponinⅥ |
Determination of Total Saponins and Asperosaponin Ⅵ in Xuduanzhuanggu Capsules |
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Authors: | Zhang Caixia Jin Linghua |
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Institution: | Zhang Caixia, Jin Linghua |
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Abstract: | Objective:To establish a method for the content determination of total saponins and asperosaponin Ⅵ in xuduanzhuanggu capsules. Methods:The content of total saponins was determined by ultraviolet visible spectrophotomet-ric method using perchloric acid as the chromogenic reagent. The content of asperosaponin Ⅵ was determined by HPLC, using Agilent C18 column (250 mm × 4.6 mm, 5 um) with acetonitrile -0.15% trifluoroacetic acid solution (29:71) as mobile phase, the detection wavelength was at 215 nm and the flow rate was 1.0 mL/min. Results: The av-erage content of total saponins was 86.3% and the average recovery was 99.88% (RSD = 1.52%). Asperosaponin Ⅵ showed a good linear relationship in the range of 64~320 μg/mL (r=0.9995) and the average recovery was 100.4%(RSD=0.771%). Conclusion: The established method is accurate and applicable to the rapid determination of the con-tents of total saponins and asperosaponinⅥin xuduanzhuanggu capsules , and can be used for the quality control of the preparation. |
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Keywords: | Xuduanzhuanggu Capsules HPLC Content Determination |
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