在体组织微循环的无标记光学微血管造影

很多生物组织的病理及血管形态与组织微循环的改变密切相关。光学相干断层成像（OCT）是一种能以微米分辨率无创获取生物结构三维图像的成像技术。光学微血管造影（OMAG）则是XqOCT数据进行处理，从而实现对生物组织血管形态的三维可视化方法。OMAG具有很高的空间分辨率，能观察到单根毛细血管，并且不需使用造影剂，其图像衬比度是基于血管内运动的血细胞后向散射产生的内源光信号。本文简回顾了OMAG技术的理论，并介绍了该技术以毛细血管分辨率在活体组织如脑、皮肤、和视网膜内的动态血流灌注成像中的应用。

Label Free Optical Microangiography of Tissue Microcirculations in Vivo

Pathologies and vascular forms of most biological tissues are clasely associated with the changes of tissue microcirculations. Optical coherence tomography （OCT） is an imaging technique capable of providing non-invasive three-dimensional （3D） images of tissue microstructure with celhdar level resohition. As a recent technological extension to OCT, optical microangiography （OMAG） is a volumetric imaging technique that is capable of producing 3D images of dynamic blood perfusion within microcirculatory tissue beds in vivo. OMAG is of high spatial resolution that is sufficient to delineate single capillary vessels, and does not require, the use of contrast agents. The OMAG imaging contrast is based on the intrinsic optical scattering signals hacksc.aue~d by moving blood cells in patent blood vessels. In this paper, I will first provide a brief review of the OMAG theory, and then present some examples of using OMAG to delineate the dynamic blood peffusion, down to capillary level resolution, within living tissues, including brain, skin, and ~tina.