脂质体在检测血清子宫内膜抗体中的应用

目的　介绍一种以脂质体作为载体检测血清中抗体的实验方法。方法用磷脂酚胆碱、神经节苷脂、胆固醇制备脂质体，在内部包裹碱性磷酸酶（ＡＫＰ），表面偶联子宫内膜抗原。取１ｍｌ上述溶液加被检血清和补体（豚鼠血清）作用后，再加入ＡＫＰ作用的底物，于４００ｎｍ处测其ＯＤ值。结果脂质体法检测健康孕妇（ＨＦＷ）和不孕症妇女（ＩＦＷ）血清的子宫内膜抗体阳性率分别为０％（０／３０）和３１．５％（３６／１１４）。与ＥＬＩＳＡ法检测的阳性率３％（１／３０）和２９．８％（３４／１１４）相近，且差别无显著性（Ｐ＞０．０５）。结论脂质体可以作为酶和抗原的载体，检测血清中的抗体。

Application of Liposome in Detecting Endometrial Antibody in Sera

Objective: To intnduce an assay with liposome as a carrier for detecting antibodyin sera. Methods Liposomes were prepared from phosphatidylcholine, ganglioside and cholesterol, and coupled endometrial antigen and entrapped alkaline phosphatase(AKP). After liposome solution lml, detecting sera 0. 05 ml and complement(guinea pig sera)0. 05ml were at 35℃ for 30min, the substrates of AKP were added. The colouring was detected in OD 400nm. Results The opitive percentage of the endometrial antibody in sera was 0% (0/ 30) in healthy fertile women (HFW) and 31. 5% (36/14) in infertile women (IFW) by using the liposome method. These results were nearly equal to the ones of ELISA, ie 3% (1/30) in HFW and 29. 8% (34/114) in IFW with no remarkable difference(P >0.05). Conclusion Liposome may serve as a carrier of enzyme and antigen detecting antibody in sera.